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...>Testing the performance of phiC31 recombinase using the register assembly construct with the attachment sites PxB.</p>
...tutive expression of phiC31 in the plant. c) Graphic representation of the construct comprised by a promoter and a terminator in opposite directions flanked by
50 KB (6,682 words) - 03:33, 2 November 2017
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<br> (2) Endolysin construct finish
<br> (5) Backbone construct-3NSII, 5NSII,AMP, B0015, and ORI (PCR: dream taq)<br>
26 KB (3,130 words) - 16:14, 28 November 2017
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...rror, so instead we performed 2-step NEB Phusion HF PCR of pSB1C3 with RFP construct</p>
<li>Performed Phusion PCR of pSB1C3 with RFP construct using psB1C3 #2 and the pKDL071 FWD/RVS primers
76 KB (12,349 words) - 20:03, 14 December 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Cross <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
199 KB (29,417 words) - 03:20, 2 November 2017
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...ldenBraid multipartite assembly reaction was performed to create a genetic construct composed by the transcriptional factor, PIF6, fused to a DNA-binding domain
...ression along time for different treatments, including the light-inducible construct (Etr8) under white-light conditions, and a weak constitutive promoter and a
51 KB (6,067 words) - 19:12, 14 December 2017
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.... The construct used for cutting <i>STE2</i> is shown in Figure 2, and the construct used for cutting <i>STE3</i> in Figure 3.
<figcaption><b>Figure 2.</b> gRNA construct for deletion of <i>STE2</i>.
253 KB (35,104 words) - 15:59, 30 October 2017
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<li style="font-family: Rubik">Designed, and began to construct, a variety of framework compatible systems, including a synthetic promoter
...moter was added to enable expression to be under the control of IPTG. This construct was then submitted to IDT for synthesis as a gBlock.
184 KB (26,744 words) - 19:53, 1 November 2017
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...moter was added to enable expression to be under the control of IPTG. This
construct was then submitted to IDT for synthesis as a gBlock.
...replacing the IPTG sensing system in the original detector module with the
construct detailed above, creating part <a href="http://parts.igem.org/Part:BBa_K2205
185 KB (26,866 words) - 22:39, 1 November 2017
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...subject, and also they get information about yeast extraction and protein construct.",,,,Consulted experts to gain more information on cyanobacteria and mathem
...ext two exchanges mainly focused on cooperation</li><li> Help BNU-China to construct a part of the gene circuit, and meanwhile, BNU-China helps with the charact
1.09 MB (163,642 words) - 17:36, 30 October 2017
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<p>Assess robustness of a GFP reporter construct in a range of environments.</p>
...ly. The Golden Gate assembly method successfully produced the GFP reporter construct, however it involved more steps and was more time consuming than Gibson ass
20 KB (2,994 words) - 21:41, 1 November 2017
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...ed split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream product
...ia in their intestines are influencing on protein nutrition. We thought to construct a pathway to synthesize amino acids from nitrogen in E.coli, introduce it i
400 KB (63,299 words) - 15:37, 15 August 2018
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...(Kittleson, 2012), which was essential both to obtain P1 particles and to construct each complete CRISPR system.</p>
engineered phagemid would need to become a P1 particle and construct whichever CRISPR system we have inserted into it within an <em>E. coli</em>
25 KB (3,973 words) - 20:43, 25 November 2017
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...lls (control) (15min) or not mixed in the presence or absence of the EL222 construct. Cell mixtures would have then be passed through a particle sizer with a ra
...fluorescence microscopy. We also included a His-Tag within all SpyCatcher construct variants to enable in-vitro analysis of complexes formed with SpyTag varian
122 KB (15,402 words) - 02:31, 2 November 2017
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<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
167 KB (25,036 words) - 21:56, 31 October 2017
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Construct standard structure of integrase int5 and int8.<br>
Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int
16 KB (1,672 words) - 13:45, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
105 KB (15,409 words) - 02:56, 2 November 2017
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...o take our chances by building a D.I.V.E.R.T. cassette as well as a helper construct (carrying the RT, FLP and the primer cassettes) according to the best of ou
...synthetic regulatory elements<sup>1–3</sup> to keep the synthesized construct short. The synthetic galactose-inducible minimal promoter we used (sequence
68 KB (10,146 words) - 17:37, 15 December 2017
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...ctroporated DNA (BRED) (Figure 1). In this technique a double stranded DNA construct containing the
...to be replaced. The host cell for recombineering of the phage and the DNA construct contains a plasmid conferring genes promoting recombination. The double-str
10 KB (1,666 words) - 23:47, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
98 KB (14,470 words) - 00:02, 2 November 2017
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<a href="#ConDPR" class="pageNavSm">
Construct Design</a>
<a href="#ConDBio" class="pageNavSm">
Construct Design</a>
55 KB (6,458 words) - 14:08, 1 November 2017
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<td>Simple, easiest to construct</td>
...espective promoter (PphlF), and a GFP reporter (BBa_K2477008). We grew our construct in different molarities of fusaric acid (0µM, 100µM, and 500 
232 KB (33,473 words) - 02:45, 2 November 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,114 words) - 00:16, 2 November 2017
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construct.<br><br>
<li>Construct half of the flip-flop structure and express it in <i>E. coli.</i></li>
170 KB (16,709 words) - 06:32, 15 December 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,037 words) - 23:41, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
159 KB (17,948 words) - 20:12, 1 November 2017
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<h2 class="textheading1" style="float:center"><u> Full Construct Testing</u> </h2>
...of GFP, the data seemed to be a bit problematic. We took a few of our full construct colonies as well as a few wild type Kanamycin resistant colonies and we ino
12 KB (1,480 words) - 02:11, 2 November 2017
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...This figure shows restriction enzyme check electrophoresis result of NrtA construct.
<h4>Endolysin Construct</h4>
29 KB (4,011 words) - 19:07, 1 November 2017
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...licated many times and matured, this allows us to have more cells with the
construct that can produce more pigment faster.
...wasted on transcribing an overly-long mRNA transcript. The melA expression
construct is the improved part that we were able to characterize the most (see <a hre
12 KB (1,924 words) - 17:29, 1 November 2017
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...luorescence was measured 20 minutes after this pH shift. The asr-mCardinal construct was inducible by change from pH 7.0 to pH 5.0 and lower but no fluorescence
...s in LPM with pH 7 overnight. For expression control of our alx-mNeonGreen construct, we inoculated 20 ml LPM with pH 7.0, 8.0 and 8.5 to an OD<sub>600</sub> of
7 KB (1,003 words) - 03:29, 2 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,127 words) - 22:23, 1 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,092 words) - 09:44, 23 November 2017
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...se). Here we performed saturation mutagenesis on the <i> araC </i> gene to construct AraC mutant library with four randomized ligand-binding pocket amino acid r
...sors and may not show sufficient specificity to xylulose. Hence, we aim to construct an alternative regulatory system which exploits the L-arabinose operon.
39 KB (6,105 words) - 16:49, 17 November 2017
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...2 regulated systems. Herein we will refer to each promoter + split protein construct as a “module”, i.e. a full AND-gate will require the presence and activ
...er for split T7 RNAP function. We utilised a preexisting T7 promoter (pT7) construct from the BioBrick registry, [http://parts.igem.org/Part:BBa_K1321338 K13213
33 KB (5,136 words) - 02:41, 2 November 2017
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<h3>New plan for assembling the cas13 construct</h3>
<h4>New plan for assembling the cas13a construct: Plan A and Plan B</h4>
656 KB (66,631 words) - 09:22, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
160 KB (18,135 words) - 23:14, 1 November 2017
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...ed AcrIIA4 from G-block and amplified pSB1C3 backbone from LacILOV-mCherry Construct in pSB1C3</td>
...ILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3</td>
7 KB (797 words) - 19:53, 14 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
59 KB (9,038 words) - 03:50, 2 November 2017
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...ngs were obtained after 24 hours of incubation. This temperature sensitive
construct was working as expected in 10β cells, elucidating higher GFP expression at
<p id="imgdescription">Figure 2: BBa_K2447014
construct which was characterised in 10β cells (incubated overnight). At higher temp
20 KB (2,876 words) - 13:25, 1 November 2017
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...work in a better understood organism and led to higher copy numbers of our
construct. </p>
<p>Our final
construct, pGUIDE, consisted of Chloramphenicol and Kanamycin resistance genes, the C
29 KB (4,511 words) - 03:55, 2 November 2017
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...">Figure 1. Graphic representation of Plant-Human genetic circuit with the construct comprised by a promoter and a terminator in opposite directions flanked by
...protein. It represents the positive control of our experiment. d) Genetic construct that allows constitutive expression of phiC31 in the plant under the contro
19 KB (2,276 words) - 03:46, 2 November 2017
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...to xylose, xylulose, ribose and mannitol. </ref> combined with a reporter construct to detect the presence of <i>C. jejuni</i>.
mtlR coding sequence was ordered as a G-block construct from IDT and was ligated into pSB1C3. As this was to be under the control o
19 KB (3,023 words) - 12:18, 12 December 2017
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...n) is added, the activity of the enzyme can be checked.<br>So far the test construct have only been implemented in silico. Also all needed primers were designed
...ed and used for a test digest with NotI. The growth of the riboswitch test construct was not properly grown. So the Golden Gate reaction and the transformation
191 KB (30,171 words) - 16:09, 15 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
56 KB (8,614 words) - 02:20, 2 November 2017
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...s indicate where a band with a molecular mass corresponding to that of the construct should be.
...re or during the purification. It wasn’t clear from our results why this construct was unstable, but we reasoned that it was too unstable for our use, so no f
20 KB (3,047 words) - 03:51, 2 November 2017
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...hecked our constructs for secondary structures and we scrambled the 14-3-3 construct to make sure that we get 4 completely different monomers, to make mutation
...of the 14-3-3 construct did not work, but the Gibson Assembly of the CT33 construct showed promising results.</h6>
34 KB (5,513 words) - 17:18, 1 November 2017
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...nhibit pλR, obstructing the promotion of tsPurple. Therefore, the working construct would supply us with a concentration of translated tsPurple dependant upon
...ration verified the success of ligation. Despite lacking the full, genetic construct, we managed to obtain viable data from the Chrome-Q system using the proof
21 KB (3,001 words) - 16:46, 16 December 2017
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...es each containing cells included to express recombinase and a measurement construct. These cells contain
a measurement construct with orthogonal target sites and give little fluorescence</figcaption>
12 KB (1,529 words) - 23:00, 1 November 2017
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...se the yields of anthocyanin when paired with the genes in our anthocyanin
construct. We have added the sequence to the registry for use in our composite part <
<p class="center">The Promoter we used for our Melanin
Construct is T7 promoter, This allows us to control the production of Melanin. This
14 KB (2,401 words) - 17:43, 1 November 2017
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<section><h3>1. Cloning of module 1:</h3><h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.
...id not get positive results for cloning, resulting in failure to clone the construct 1.
12 KB (1,800 words) - 03:21, 2 November 2017
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<section><h3>1. Cloning of module 1:</h3><h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.
...id not get positive results for cloning, resulting in failure to clone the construct 1.
12 KB (1,743 words) - 04:15, 14 December 2017
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...d to turn the protein and its required guide sequence into a BioBrick. The
construct was delivered in three fragments that had to be assembled with the backbone
...extracted from <a href="#references">Gootenberg et al. 2017</a>. This DNA
construct (7.7 kb), was ordered in three separate parts, denoted part 1 (2.5 kb)
102 KB (13,845 words) - 16:15, 15 December 2017
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...eful vector pPIGBACK for microalgae transformation. pPIGBACK is a powerful construct with NSII, AmpR, double terminator, ORI, and PrbcL, and it is able to embar
<!--construct-->
13 KB (1,734 words) - 19:39, 1 November 2017
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<h2 class="major">Construct Model</h2>
...d COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composed of fusion proteins as well as split luciferase fragments (COX-2 -
78 KB (7,559 words) - 15:53, 1 November 2017
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.... Suddenly I came up with an interesting idea that what if the scaffold is construct by protein. Then I realized that scaffold is such a regular network that we
... So now the problem is how do we construct the scaffold. We knew that there was many protein self-assembly system. Usu
22 KB (3,229 words) - 09:18, 29 August 2017
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<input class="tealButton" type="button" value="Construct Design" onclick="window.location.href='#anchor3'" />
<input class="tealButton" type="button" value="Validation Construct" onclick="window.location.href='#anchor7'" />
25 KB (3,716 words) - 02:10, 2 November 2017
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...s (Center Point and Binding Partner) are connected and form the <i>Binding Construct</i>. There is a <i>Center Point</i> and at this Center Point, four Binding
<li><span>Of the Scaffold Construct</span></li>
5 KB (769 words) - 21:13, 1 November 2017
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Through simulations of our intended construct with parameters defined a priori, we were able to draw some conclusions as
<h3 class="section-heading">Control construct design</h3>
12 KB (1,643 words) - 01:46, 2 November 2017
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<a class="item passlink" data-passage="consexp">Construct Expression Vector for NzFE</a>
<td>easy to construct</td>
21 KB (2,679 words) - 13:26, 15 December 2017
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● Build up deinking enzyme construct<br>
● Transform construct into yeast<br>
12 KB (1,855 words) - 03:13, 16 December 2017
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...RNA from the HTT115 strain witouth the siRNA
construct and with the siRNA
construct (BSLA-AWD) with different hybridization oligos and treatments in a 12% no-d
<p>Figure 2. RNA from the HTT115 strain with the siRNA
construct (BSLA-SOD, BSLA-WNT and BSLA-RACI) with different hybridization oligos and
42 KB (4,985 words) - 03:59, 2 November 2017
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<td>Construct for Curvacin A expression</td>
<td>Construct for Enterocin A expression</td>
19 KB (2,225 words) - 19:40, 1 November 2017
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...e to subclone the immunity protein 2 into the tryptophan regulatory system
construct and perform proliferation tests. Unfortunately, due to time constraints, we
...uccess rate of a study utilizing machinery for genome integration. The RFP
construct was cloned into the UPO-Sevilla 2011 plasmids (<a target="_blank" href="htt
48 KB (6,966 words) - 15:28, 15 December 2017
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...contain EutSMNLK, however we were unable to obtain pure plasmid from this construct containing all microcompartment subunits as the transformed colonies became
...Tris-glycine SDS page gels of soluble and insoluble Eut S, MN, SMN and LK construct proteins. (-) indicates
12 KB (1,634 words) - 03:48, 2 November 2017
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...olor: #808080; font-size: 14px;'>Figure 1: Schematic of a generic reporter construct used to test degradation rates. Analogous constructs were built with sfGFP
...tructs (Figure 3a). Further, if you plot the gene expression speed of each construct against its degradation rate then the shape of the curve resembles that of
19 KB (2,797 words) - 03:07, 15 December 2017
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<li><b>Fer/Hyd</b>- Fer and Hyd as described above in a new biobrick construct.</li>
...i> and <i>hyd G</i>, coding all three maturation enzymes in a new biobrick construct.</li>
55 KB (9,025 words) - 13:09, 31 October 2017
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...atase that converts the enoyl-CoA into (R)-hydroxybutyrate (Lu, 2003). Our construct also includes the <i>phaC1</i> gene from <i>Pseudomonas aeruginosa</i>, enc
<h2>Genetic construct</h2>
7 KB (1,176 words) - 01:03, 2 November 2017
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...last monomer, leading to a functional valency of 3. The structure of this
construct is shown schematically in <a href="#Figure_1">Figure 1.</a>
..., leading to our second
construct, which has thus a valency of 4. The CT33
construct is schematically shown in <a href="#Figure_2">Figure 2</a> below.
7 KB (1,092 words) - 22:03, 11 December 2017
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...te-temperature cascaded system with GFP reporter. Characterisation of this construct was conducted with varying phosphate concentrations under two different tem
...led plan: Culture the transformed cells ( the temperature sensitive system construct, is transformed into MG 1655) from glycerol and incubate for 36 h in 30 deg
17 KB (2,425 words) - 16:34, 1 November 2017
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...g a VCP1-GFP-VCP2 construct and attempting to get the algae to express the construct. <br> <br>
.../2017/b/be/UConn_vcp1gfp_gel.JPG" class="vcp1-gfp"> <br> First, a VCP1-GFP construct was assembled through digesting the GFP out of its original backbone and th
15 KB (1,886 words) - 03:25, 2 November 2017
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...d series of anderson promoters next to the RNA I gene and then placed this construct next to RNA II (RNA II-Anderson-RNA I).</p>
...le copy number system. We first had to make sure that at least part of our construct is well characterized and to so we chose the rhamnose promoter from the bio
58 KB (8,538 words) - 14:52, 26 November 2017
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recombinase expression construct in pET28b, a medium copy plasmid (10~15), another one being Promoter-LoxP-T
...mediate the recombination between the two target sites on the measurement construct, excising the transcriptional terminator. This leads to the expression of R
79 KB (9,298 words) - 20:57, 1 November 2017
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The construct is long (1,029 bp), so we got only part of it sequenced due to using only o
...k sample no. 13 from the check-up digestion (Figure 8) for sequencing. The construct is long (1029 bp), so we got only
53 KB (6,877 words) - 20:55, 11 December 2017
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<cite>Figure I: Scheme of the BBa_K2425000 construct. It is important to know that the number of bases pairs is just the coding
...f METE gen (Helliwell et al.,Plant physiology, 165(1), 388-397, 2014). The construct has 574 bp and contains the 5’ UTR and the sequence of the promoter. All
17 KB (2,489 words) - 01:44, 16 December 2017
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...of the highly sensitive parameter in the design. As the original phosphate
construct <a href="http://parts.igem.org/Part:BBa_K116404">BBa_K116404</a> (
construct
5 KB (689 words) - 03:09, 2 November 2017
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.... The second step was the preparation of a pTet-GFP/RFP and a pLac-GFP/RFP construct to test these promoters in L. lactis. From next week progress for this part
...ad><tr class="tableizer-firstrow"><th>Backbone</th><th>construct A</th><th>construct B</th><th>Product name</th></tr></thead><tbody>
50 KB (7,945 words) - 03:35, 2 November 2017
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Each construct was sent for sequencing with primers VR and VF
...<li>RPA of TEV could not produce the whole (~1300 bp) construct but would produce amplicons of shorter size (100-500 bp) which was anticipa
380 KB (41,086 words) - 11:45, 1 November 2017
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Each construct was sent for sequencing with primers VR and VF
...<li>RPA of TEV could not produce the whole (~1300 bp) construct but would produce amplicons of shorter size (100-500 bp) which was anticipa
380 KB (41,105 words) - 02:27, 2 November 2017
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.../static.igem.org/mediawiki/2017/2/2b/XylRproofofconcept.jpg" alt="xylR-GFP Construct" style="width:600px;height:175px;"></center>
...nts, we eventually arrived at construct BBa_K2451013, depicted above. This construct displays promising results for m-xylene induction. The xylR coding sequence
46 KB (5,986 words) - 02:38, 2 November 2017
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..."></div><div>w that iron had no effect on the production of amilCP in this construct and we compared that to the co-transformed bacteria (plasmids 1+2). Again,
...rmed cells (i.e. cells with the FUR repressed construct and LacI repressed construct) within 24 hours, although to a lesser extent than cells which lacked the f
12 KB (1,980 words) - 18:53, 1 November 2017
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<h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.</h4>
<h4>Construct 2 --> P<sub>T7</sub> - RBS2 - Chromoprotein II - RBS3 - tetR - terminator3.
11 KB (1,384 words) - 04:14, 14 December 2017
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<p>When designing this construct, we found a paper by Chen et al. that determined the optimal spacing betwee
...the production of varied gas vesicle phenotypes. Expression of the gvp3.0 construct is under control of the lac promoter (pLac), allowing inducible expression
9 KB (1,385 words) - 03:58, 2 November 2017
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...find out the best combination of vector and E.coli strain for this sensing construct.
3 KB (501 words) - 04:56, 16 December 2017
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<b>Figure 3:</b> construct design for 2 Dronpa domains
...y altering its conformation. To test this hypothesis we made the following construct:
21 KB (2,411 words) - 03:11, 16 December 2017
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...to time constraints of the parts submission deadline, we were not able to construct a new biobrick compatible hCas9 operon. We encourage next iGEM teams to pic
As mentioned earlier we were not able to construct the biobrick compatible hCas9 operon. So, the experiment described next was
25 KB (3,964 words) - 03:48, 2 November 2017
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var reservedEventNames = /^(?:change|reset|teardown|update|construct|config|init|render|complete|unrender|detach|insert|destruct|attachchild|det
....error( 'Cannot use reserved event names (change, reset, teardown, update, construct, config, init, render, unrender, complete, detach, insert, destruct, attach
1.96 MB (302,682 words) - 17:51, 24 October 2017
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...<i>Clostridium thermocellum</i>. This part could be used with any other construct to bind to cellulose.
...l peptide in form of a fusion protein accompanied with His6x-Smt3 tag. The construct can be purified with commercially available and widely used affinity chroma
18 KB (2,363 words) - 20:51, 15 December 2017
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...n later constructs and successfully isolated those. Digested all parts for construct 1 so far and gel purified the adequate bands.
Transformed cells with construct 1 and created glycerol stocks from those. Prepared a protocol for the gener
7 KB (1,157 words) - 13:03, 31 October 2017
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.... The second step was the preparation of a pTet-GFP/RFP and a pLac-GFP/RFP construct to test these promoters in L. lactis. From next week progress for this part
...ad><tr class="tableizer-firstrow"><th>Backbone</th><th>construct A</th><th>construct B</th><th>Product name</th></tr></thead><tbody>
39 KB (6,407 words) - 01:03, 2 November 2017
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...icin. Successful cloning was confirmed by gel electrophoresis, and the DNA construct was sent for sequencing. Whilst waiting for sequencing results, we began ch
...o do this, we used the already existing BioBrick BBa_I6211, containing the construct osmosensitive promoter-RBS-YFP-double terminator in the pSB1C3 backbone. As
16 KB (2,484 words) - 19:09, 15 December 2017
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...RNA from the HTT115 strain witouth the siRNA
construct and with the siRNA
construct (BSLA-AWD) with different hybridization oligos and treatments in a 12% no-d
<p>Figure 2. RNA from the HTT115 strain with the siRNA
construct (BSLA-SOD, BSLA-WNT and BSLA-RACI) with different hybridization oligos and
14 KB (1,654 words) - 01:36, 2 November 2017
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...uorescence detection assay utilizing green fluorescent protein (GFP)-fused construct to BBa_I14034 promoter part.</p>
This construct is produced by combining BBa_114034 promoter part and a GFP coding sequence
3 KB (469 words) - 03:58, 2 November 2017
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...roceeded to insert the T2A-P2A based display vector into our final ToleGen construct.
Our final experiment was to test the ToleGen construct we created <strong>(figure 5)</strong>.
20 KB (2,612 words) - 02:51, 2 November 2017
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...ow exchanging of the separate parts. As a result, we decided to divide our construct into 3 gBlocks, which could be connected to each other and placed in a pET2
...e large gBlocks and large amount of inserts, so we decided to redesign our construct, by dividing it into two gBlocks: one containing the first two monomers and
8 KB (1,272 words) - 13:50, 10 December 2017
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...xpected size band of ~3.5 kb. <strong>C:</strong> Results of <em>VanA</em> construct amplification via PCR using amplifying primers at four temperatures, 66°
...hite MoClo colonies were checked for correct insertion of the <em>KPC</em> construct via digestion with AvrII (A). All bands were of the expected size and uncut
9 KB (1,446 words) - 23:48, 1 November 2017
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<input class="tealButton" type="button" value="Validation
Construct" onclick="window.location.href='#anchor4'" />
<p class="text12j">Both the tRNA<sup>phe</sup>-MS2 aptamer
construct and MS2BP were overexpressed individually in <i>E. coli</i> BL21-Gold (DE3)
20 KB (2,975 words) - 03:38, 2 November 2017
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<p>To characterize LacILOV, cells containing LacILOV-mCherry construct were grown under a consistent light intensity and the fluorescent output wa
...tivity of the LacILOV mCherry construct, the assays for assessing reporter construct use the same hardware devices for delivery of light and dark conditions. Th
16 KB (2,418 words) - 19:50, 14 December 2017
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...ry tag has on enzyme activity. Results from this will aid how we build the construct to target the enzyme into the microcompartment at a later stage.</p>
<th>Construct</th>
26 KB (3,896 words) - 01:44, 2 November 2017
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...on developing a proof of concept for the Chrome-Q and assembling a genetic construct to use the Chrome-Q to quantify data.
Several weeks were devoted to construct pλR LacI R011 RFP in the NEB 10-beta E. coli strain, with individual parts
26 KB (3,923 words) - 17:16, 12 December 2017
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...d sequencing results from Tri-I Biotech confirmed that our final <i>pR</i> construct is correct.
...<b>Figure 2-2 Construct expressing PR. </b> Our construct includes a strong promoter, strong RBS, the <i>pR</i> ORF, and a double ter
19 KB (1,932 words) - 13:20, 31 October 2017
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<input class="tealButton" type="button" value="Validation
Construct" onclick="window.location.href='#anchor3'" />
<p class="text12j">Both the tRNA<sup>Phe</sup>-MS2
construct and MS2BP were expressed individually in <i>E. coli</i> BL21-Gold (DE3) cel
9 KB (1,447 words) - 02:13, 2 November 2017
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...se the yields of anthocyanin when paired with the genes in our anthocyanin construct. We have added the sequence to the registry for use in our composite part <
<h2><b> Melanin Construct</b></h2>
10 KB (1,730 words) - 17:37, 1 November 2017
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...engineer <i>E. coli</i> to synthesize PHB. We designed a pET29b(+)-phaC1J4 construct to allow our engineered <i>E. coli </i> to employ the beta-oxidation pathwa
...nfirm the ability of <i>E. coli</i> transformed with our pET29B(+)-phaC1J4 construct to produce PHB, we grew O/N cultures for 24 hours and induced them with 0.1
34 KB (5,337 words) - 04:28, 16 December 2017
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...sequence is identified as integral in determining whether the single chain construct has activity.</h4><br>
...ney_Australia/Patent_Law">patents</a>. With this in mind - we designed our construct to also have 12 amino acids.<br>
66 KB (8,543 words) - 03:14, 2 November 2017
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...ttps://static.igem.org/mediawiki/2017/d/d1/T--Bielefeld-CeBiTec--composite-construct.png">
...fot the mRFP transcript is downstream the CDS of the gene of interest. In construct 2 the CDS of the mRFP is downstream a T3 promoter and the CDS coding for th
27 KB (4,095 words) - 01:18, 2 November 2017
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...titutive promoter psaD (and its respective terminator) The detail of these
construct are shown as follows in the next image:</p>
<p id="FigureText">Fig. 1: Scheme that shows the fusion protein
construct to make the first transformation of <i>C. reinhardtii</i>.</p>
8 KB (1,220 words) - 00:35, 30 October 2017
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In order for our construct to work, it had to be transfected into HEK2935, a mammalian cell line. RNA
We transfected our construct (BBa_K2340000) with a guide RNA plasmid (BBa_K2340001 - BBa_K2340011) (1:2
21 KB (2,470 words) - 04:21, 16 December 2017
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...e our chances of successful assembly. We did succeed in inserting the melA construct into pJET (Figure 1).
...l of melA_pJET digested with EcoRI and PstI. Plasmids 1 and 3 had the melA construct successfully insert into pJET without disruption of the cut sites.</i></p>
8 KB (1,262 words) - 13:54, 1 November 2017
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In addition, the tested construct was approximately 1500 bp long, which is also quite far from RPA's optimum
construct of 200 bp, one larger in size and less intense and one smaller in size and
11 KB (1,840 words) - 21:57, 31 October 2017
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...titutive promoter psaD (and its respective terminator) The detail of these construct are shown as follows in the next image:</p>
<cite>Fig. 1: Scheme that shows the fusion protein construct to make the first transformation of C. reinhardtii.</cite>
9 KB (1,363 words) - 03:56, 2 November 2017
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...l peptide in form of a fusion protein accompanied with His6x-Smt3 tag. The construct can be purified with commercially available and widely used affinity chroma
The construct can be purified with commercially available and widely used affinity chroma
10 KB (1,538 words) - 20:53, 15 December 2017
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<p class="PP">This year, we aim to construct a diseases-monitoring system for automatic agriculture (AA) in which biolog
...ce<sup>[2]</sup>. What’s more, DAPG can also serve as signal molecule to construct a transcriptional switch that is similar to Tet on/off system<sup>[3]</sup>
31 KB (3,735 words) - 15:38, 1 November 2017
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...;\n }\n if (arguments.length < 1) {\n throw new TypeError(\"Failed to construct 'SockJS: 1 argument required, but only 0 present\");\n }\n EventTarget.ca
...the same order.\n // 5. Return the result of calling the [[Construct]] internal\n // method of target providing args as the arg
932 KB (107,677 words) - 07:59, 24 October 2017
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...;\n }\n if (arguments.length < 1) {\n throw new TypeError(\"Failed to construct 'SockJS: 1 argument required, but only 0 present\");\n }\n EventTarget.ca
...the same order.\n // 5. Return the result of calling the [[Construct]] internal\n // method of target providing args as the arg
932 KB (107,677 words) - 08:00, 24 October 2017
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...and plated. Preliminary test of RFP expression with BBa_K1966004 (2016 Pu construct)
- Colony PCR on xylB construct – success (right)
66 KB (8,136 words) - 00:10, 2 November 2017
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...aled for both species. Only the <i>gambiense</i> gene was used for further construct building. It was gel purified and digested with both KpnI and SacI. The bac
The construct building was repeated, since it failed in week 3. First, the ligation was r
8 KB (1,182 words) - 12:59, 31 October 2017
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<p><b>Superior experiment:</b> Gaining more of the construct pSB1C3-AraC-chiA</p>
<li style="font-size: 15px;"> The mutation still remains in the construct</li>
96 KB (13,160 words) - 21:18, 1 November 2017
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* IPTG Induction of BL21 promoter construct
* Transformation of BL21 with pick 4 of promoter B1C3 construct
10 KB (1,332 words) - 17:46, 31 October 2017
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...P fluorescence obtained from BL21(DE3) with the pETDuet-GST-f-p+pET28a-GFP construct cultured in LB medium (10X40).</span>
...fluorescence obtained from BL21(DE3) with the pETDuet-GST-f-p+pET28a-EGFP construct cultured in MOPS medium (10X40).</span>
26 KB (3,823 words) - 18:49, 1 November 2017
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...ts were run on a gel and gel purified in order to isolate the promoter-RFP construct. These constructs were then ligated using T4 ligase into pCAMBIA-MCS which
..., however there were colonies on the LB+Kan plates of <i>virB1</i>-pCAMBIA construct for both plasmid backbones, BBa_K1357010 and BBa_J04650.
19 KB (2,909 words) - 03:17, 2 November 2017
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...ing. The construct was tested in HEK293FT cells, targeting five genes. The construct was transfected concurrently with a GFP donor.</p>
...ge of the knock-in event for GAPDH gene. The data were normalized to empty construct. Error bar is +- s.d.
20 KB (3,198 words) - 03:53, 2 November 2017
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...he vector and insert, assembling the construct, transforming the assembled construct into DH5α cells, and verifying the assembly success via colony PCR and seq
...the DNA polymerase and DNA ligase lysate to assemble their PelB-saCas9-His construct using optimal ratios that we established. The success of the assembly would
8 KB (984 words) - 03:44, 2 November 2017
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...LuxR will result in a significant amount of GFP production. The goal is to construct a variation on this part that no longer has spontaneous activation, but can
...sults support the strategy of using aiiA to tune the sensitivity of the QS construct and indicate that, in principle, the functionality we want to engineer can
20 KB (2,280 words) - 21:40, 1 November 2017
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<li>Cells engineered with our Display construct present epitopes on their membrane MHC complexes.</li>
We came up with two possible ways to adequately test our construct:
18 KB (2,317 words) - 01:45, 2 November 2017
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Standard Genetic Construct for Pigment Production
...i> ribosome binding site (RBS) B0034 (with the exception of the zeaxanthin construct, which uses <i>Pantoea ananatis</i> native RBSs), the coding sequences for
12 KB (1,702 words) - 15:49, 1 November 2017
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...eriment, overnight cultures of MG1655 ΔLacI cells containing the reporter construct were diluted and further cultivated in 5 mL of culture in 15 mL falcon tube
...s with the same CRISPRi system and the anti-CRISPR protein AcrIIA4. If our construct functions as expected, the anti-CRISPR protein should bind to pdCas9 and pr
22 KB (2,497 words) - 18:54, 14 December 2017
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...n in lanes 2-6 show clean single bands that correspond to their respective construct sizes (Cytoplasmic Winsulin: 306bp, Ecotin Winsulin: 867bp, YncM Winsulin:
...n of Ecotin insulin due to time constraints, we did test our YncM Winsulin construct. YncM is an N-terminal tag which, when fused to a protein of interest and e
20 KB (2,972 words) - 23:39, 1 November 2017
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...parts listed below have all been functionally verified, and can be used to construct the systems
the validity of our measurement construct for testing if two recombinase sites are orthogonal. Indeed, it
26 KB (3,167 words) - 10:44, 1 November 2017
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...se the yields of anthocyanin when paired with the genes in our anthocyanin
construct. We have added the sequence to the registry for use in our composite part <
<p class="center">The Promoter we used for our Melanin
Construct is T7 promoter, This allows us to control the production of Melanin. This
8 KB (1,257 words) - 15:53, 1 November 2017
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<li>Make construct mix:<br>
<li>Pipette 2000 µl of the construct mix dropwise to the HEK293T cells</li>
30 KB (3,843 words) - 02:17, 2 November 2017
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<p>To begin with, we gave them
construct suggestions, communicated with their team adviser. In 24/3/2017, we were in
<p>1. NKU asked us to help them
construct a plasmid. Using pEX18Gm as the supporter, we ought to added Lyase gene seq
18 KB (2,589 words) - 16:09, 16 October 2017
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<h2><b> Melanin Construct</b></h2>
This construct allows us to make Melanin out of L-Tyrosine.
8 KB (1,277 words) - 17:41, 1 November 2017
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and were pleased to see that it worked well. This construct we called the
Figure A as a ssDNA molecule and tested it in our lysates. This construct
12 KB (1,870 words) - 02:18, 2 November 2017
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<li><span>Scaffold Construct (14-3-3 with GFP) + Binding Partner (CT33 with Strep-tag®II and mCherry) +
<li><span>Scaffold Construct (14-3-3 with GFP) + Binding Partner (CT33 with Strep-tag®II and mCherry) +
7 KB (1,058 words) - 13:47, 10 December 2017
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...2436001) was cloned successfully and submitted to the registry. The lipase construct was not cloned successfully. Our constructs were designed as follows.
...onstruct contains the chlorogenate esterase coding sequence, and the other construct has the lipase coding sequence.
6 KB (849 words) - 19:44, 19 November 2017
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...<sup>(5)</sup> or VP64<sup>(6)</sup> as transcription regulating domain to construct silencing- or activating-form of SynTFs, named SynTF(S)s or SynTF(A)s. Thei
...ensive usage in genome editing<sup>(10)</sup>. We applied this strategy to construct tunable SynTF-SynPro pairs. With more RE repeats, our SynTF has stronger in
28 KB (4,314 words) - 14:20, 14 December 2017
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<p class="PP">This year, we aim to construct a diseases-monitoring system for automatic agriculture in which hardware an
...Recently, it is demonstrated that 2,4-DAPG can serve as signal molecule to construct a transcriptional switch that is similar to Tet on/off system.</p>
41 KB (5,261 words) - 15:13, 1 November 2017
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...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
8 KB (1,193 words) - 00:30, 2 November 2017
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...s which make AI-2, such as DS941 <i>E. coli</i>, when compared to the same construct in cells that don’t make AI-2, such as DH5α <i>E. coli</i>. The results
...et up from single colonies of strains containing P<sub>lsrA</sub> promoter construct.
15 KB (2,539 words) - 21:46, 1 November 2017
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Jurkat and HEK293T cells were transduced with
construct containing the suicide gene <i>herpes simplex virus 1</i> thymidine ki
<p><strong>Figure 11: Cloning strategy of knockdown
construct.</strong><br>
64 KB (8,680 words) - 03:27, 2 November 2017
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– Positive colonies carrying construct in tetracycline backbone from last week were re-streaked to check for viabi
...screening by digestion was done with modifications as the plasmids and the construct were of similar size therefore needed better resolution. <br>
28 KB (4,144 words) - 21:46, 1 November 2017
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<p>After achieving getting the signal for a particular VOC, we aim to construct logical gates and circuits by using CRISPRi system on plasmids to create we
...ration in their urine samples compared to healthy people. [4] The designed construct aims to get a correlated GFP signal with formaldehyde concentration.
27 KB (4,457 words) - 01:26, 2 November 2017
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Fragments obtained from IDT to construct our plasmid designs were not suitable for Gibson Assembly. Therefore, we or
...box shows amplification of a single fragment containing all the genes for construct K2268006 (MazF and cI repressor). The second yellow box shows the various f
7 KB (527 words) - 01:28, 2 November 2017
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...n any modifications (except for a substituted glycine on the A chain), our construct is outside the patent<o:p></o:p></span></p>
...ang="EN-AU" style="font-size:14.0pt;font-family:Quicksand">In our sequence construct </span><span lang="EN-AU" style="font-size:14.0pt;font-family:Quicksand;mso
294 KB (38,580 words) - 22:35, 22 November 2017
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...the fact that we were a lacking some reagents was key for us to decide to construct a constitutive circuit. At last, what we were trying to prove at this point
...uences at the NCBI database better characterized. Therefore, we decided to construct two new Biobricks with better experimental backgrounded proteins that will
7 KB (1,195 words) - 00:13, 28 October 2017
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...the parts registry page from last year was updated for clarity on the full construct design.
11 KB (1,383 words) - 03:52, 2 November 2017
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<p id="
CONSTRUCT-1-Part-BBa_K1163103-0"><img alt="File:Judd UK Updated Constructs.jpeg" src=
<h2 id="
CONSTRUCT-1-Part-BBa_K1163103-0">
CONSTRUCT 1: Part BBa_K1163103</h2>
4 KB (558 words) - 17:30, 1 November 2017
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...he fact that we were a lacking some reagents was vital for us to decide to construct a constitutive circuit. At last, what we were trying to prove at this point
...uences at the NCBI database better characterized. Therefore, we decided to construct two new Biobricks with better experimental backgrounded proteins that would
15 KB (2,493 words) - 03:40, 2 November 2017
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...(<a href="http://parts.igem.org/Part:BBa_K2447014">BBa_K2447014</a>). The
construct was functioning properly and elucidating higher GFP expression only when th
...(<a href="http://parts.igem.org/Part:BBa_K2447000">BBa_K2447000</a>). The
construct was functioning properly and elucidating higher GFP expression only when th
7 KB (963 words) - 11:59, 1 November 2017
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<p>Succeed
construct BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013.</p>
<p>
Construct BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_
10 KB (1,213 words) - 19:22, 1 November 2017
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...ffect. We want to find out if it is really the case in our warriors, so we
construct our warriors and carry out the killing test. <br><br></div>
<div class="myPara" > 1.
Construct our warriors and beggars by co-transformation. (the gene circuits are as fo
15 KB (2,229 words) - 15:55, 1 November 2017
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In order to test the mechanism, a strain containing a construct expressing GFP, LuxI and LuxR under the pLux promoter and the aiiA molecule
...<b>Figure B:</b> Gene sequence depicting the Inducible quorum sensing construct whose decrease in fluorescence is induced under the addition of anhydrotetr
64 KB (7,345 words) - 22:30, 1 November 2017
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...in (HBG) intron 2. We used this split mKate 2-exon
construct as a reporter
construct that we used to determine if our dCas13 or Ms2 systems were successful in s
<p>The figure above represents our
construct. The sequence in between the exons represents HBG intron 2. The 3' splice s
4 KB (655 words) - 01:31, 2 November 2017
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...Master Mix protocol. 7 colonies from each plate were run along with a RFP Construct postitive control and milliQ water negative control.
...Colonies from each 9/30/17 golden gate reaction were run along with a RFP Construct postitive control and milliQ water negative control.
64 KB (11,762 words) - 23:39, 1 November 2017
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<h2>Construct</h2>
...that the parts could be analyzed relative to other systems. Generally, the construct consisted of a constitutive promoter, the riboswitch - which includes a rib
9 KB (1,391 words) - 02:48, 16 December 2017
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...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
7 KB (951 words) - 23:51, 1 November 2017
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...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
7 KB (951 words) - 02:34, 2 November 2017
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...berg et al. 2017</a>. We did this in parallel with the assembly of our own construct. The activity of Cas13a was tested with fluorescence activity assays, which
...following unfoldables. See Figure 2 for a schematic overview of the entire construct.</p>
82 KB (10,812 words) - 22:11, 14 December 2017
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...ure 1. Scheme of the 3D printed device for the delivery of the HDT genetic construct with the use of bacteriophages. (A) Soft-impermeable membrane that allows p
...ualization of the 3D-printed prototype for the delivery of the HDT genetic construct with the use of synthetic bacteriophages (A) Closed tube with the Piercing
11 KB (1,735 words) - 02:40, 2 November 2017
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...ve types of culture at 37C. This is promising because it tells us that our construct has no negative impact on cell growth at working conditions. In theory, pro
...future applications of our design. In the future we would incorporate our construct into both pathogenic strains such as MRSA and non-pathogenic strains used i
19 KB (2,518 words) - 23:43, 1 November 2017
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...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
...e estimation of expression of their protein of interest. In addition, this construct will give an added advantage to tune the expression of their protein of int
31 KB (4,088 words) - 00:32, 2 November 2017
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1 KB (169 words) - 20:30, 1 March 2018
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The first construct containing the genes required for the light-induced dormancy was designed a
...ite between the LacI-regulated, lambda hybrid promoter and this particular construct under the tested conditions had formed a hotspot for transposons. In the li
384 KB (54,129 words) - 02:00, 2 November 2017
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...is. The graph below shows evidence for mf-Lon induced toxicity. The J23100 construct does not possess a pdt and fluorescence decreases despite that no GFP is ge
...se.) We started thinking about how exactly we'd want to design an mScarlet construct. Planning to clone mScarlet versions of J23100 + pTet parts (3K3 + 1C3).</d
30 KB (4,512 words) - 03:20, 2 November 2017
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...attached at the end of the tsPurple construct. BBa_K2353000, the tsPurple construct without any degradation tag, shows the baseline expression of tsPurple as a
11 KB (1,436 words) - 03:40, 2 November 2017
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...ssembled because it is the same promoter that is on the second part of our construct. Due to this, we moved back to pλR LacI and inoculated liquid cultures fro
In order to troubleshoot our pλR LacI construct, we decided to attempt to recreate the part from the iGEM Distribution Kits
17 KB (2,322 words) - 23:54, 1 November 2017
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The first construct containing the genes required for the light-induced dormancy was designed a
...ite between the LacI-regulated, lambda hybrid promoter and this particular construct under the tested conditions had formed a hotspot for transposons. In the li
424 KB (60,406 words) - 11:01, 20 November 2017
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...nstruct plasmids were all tested. The OD reflects the fluorescence of each construct tested in the presence of sugar. Again, ribose and sorbitol show the greate
...analysis compared three BL21 (DE3) colonies before (Pre) and after (Post) construct expression was induced with IPTG. Marker used was 1kb+ ladder (Invitrogen)
9 KB (1,325 words) - 01:43, 2 November 2017
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...he combination of up to 7 different transcription units yields a multigene
construct. </p>
<br><i><div style="font-size:80%;"> Figure 2: Different levels of
construct assembly (P: promotor, CDS: coding sequence, T: terminator)</div></i><br><b
57 KB (7,992 words) - 20:08, 1 November 2017
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<p>To quantify the amount of PHB made by our phaCBA
construct, we made 9 overnight cultures of <i>E. coli</i> BL21(DE3) that contained th
<p>The results showed that PhaJ was being expressed for our pET29b(+)-phaCJ
construct in the insoluble phase. After trouble-shooting our experiment, we decided t
189 KB (30,380 words) - 06:23, 16 December 2017
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...936003): Part of a red light-controlled synthetic genetic switch. Chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650)
...etic switch. Designed module for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-1
26 KB (3,213 words) - 02:23, 2 November 2017
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...n of PhiC31 actuation in plant-human genetic circuit. A) Register assembly construct composed by 35s promoter inverted sequence and Mtb terminator flanked by tw
...">Figure 3. Graphic representation of Plant-Human genetic circuit with the construct comprised by a promoter and a terminator in opposite directions flanked by
41 KB (5,363 words) - 03:07, 2 November 2017
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...ation strategy, we plan to synthesize the remaining tRNA in our expression construct for submission into this collection.</p>
...><b>Validation:</b> Finally, we have also included our measurement control construct for the complete TX-TL system. This part expresses a RNA Spinach tagged EYF
24 KB (3,354 words) - 02:38, 2 November 2017
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...e <i>vox-ura3-terminator-vox</i> structure above. Therefore, it is easy to construct because we only need to change the <i>ura3</i> gene to the <i>yEmRFP</i> ge
...conveniently. To show the function of Mating Switcher more intuitively, we construct this<i> RFP</i> system to be a characterization.
52 KB (7,750 words) - 03:12, 2 November 2017
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All plasmids except pSB1C3-PtNTT2 were tagged with the same cMyc-GFP construct for imaging and western blots. cMyc was used as a linker to allow the targe
...-cMyc-GFP with a TAT signal peptide. No bands can be observed for the full construct, but a very weak band can be seen between 55 and 70 kDa for the fusion prot
56 KB (8,532 words) - 20:58, 29 October 2017
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At first, we designed our construct carrying our synthesized single guide RNA (sgRNA), which is essential for t
The PCR-construct responsible for the homologous recombination, and therefore the <i>cod</i>A
30 KB (4,719 words) - 03:58, 2 November 2017
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<h3> construct a biobrick </h3>
<h3> construct a biobrick </h3>
131 KB (18,470 words) - 03:56, 2 November 2017
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...choice of a basic strain of microorganism producers for expression of the
construct created.</p>
<p>When it was time to clone our
construct in the pSB1C3 vector that we had received from iGEM our team faced some dif
11 KB (1,746 words) - 12:18, 30 October 2017
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...the three functional 14-3-3 monomers. We also described that the Scaffold construct would be a tetramer, but because we mutated the last pocket, the pocket has
...ude a Strep-tag®II. The CT33 site can bind in the pockets of the Scaffold construct filled with inducer and the Strep-tag®II site can bind to Strep-Tactin®XT
4 KB (563 words) - 13:48, 10 December 2017
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<p>LB,
construct, Tet</p>
<p>-
Construct primers for G-blocks</p>
23 KB (3,938 words) - 20:58, 31 October 2017
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...he effect of different concentrations of luciferin on <i>Agrobacterium</i>-
construct infiltrated leaf disc samples. This can be seen on the <a href="https://201
2 KB (314 words) - 11:45, 30 October 2017
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...s why we could not synthesize all of the genes as a single
construct. Each
construct also contained promoter, ribosome-binding sites and the necessary homologie
<div style="padding-bottom:3%"> A
construct with beta-carotene hydroxylase gene <i>crtZ</i> and lycopene cyclase gene <
14 KB (1,977 words) - 21:23, 1 November 2017
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...l part of the dCas9QC with a gBlock containing the VRER mutations. The new construct was analyzed by sequencing the last part containing the gblock (figure 4).
...). These gRNA's were inserted into the plasmid pSB3C5 containing a pLacGFP construct as well. For dCas9 and dCas9VRER expression those parts were put into a pBa
21 KB (3,475 words) - 03:16, 2 November 2017
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Our CRISPR/dCas13a
construct functions as an “mRNA localization device” to investigate the fate of
<img src="https://static.igem.org/mediawiki/2017/d/d0/T--Kent--
construct.png">
15 KB (1,712 words) - 00:26, 16 December 2017
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...hich HheC-W249P achieve the best results, so We finally used the mutant to construct the plasmid.
...yme ratio of 1: 2: 4, so although EchA is expressed in the plant, we still construct the gene, hoping to let the plant overexpress the enzyme to a certain exten
20 KB (2,797 words) - 21:09, 30 October 2017
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<li> Make a label (clone ID # and date) for the construct.</li>
...<li> Determine the location (-80°C tower #, box #, row #) of the construct.</li>
28 KB (3,495 words) - 22:23, 1 November 2017
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We tested our
construct using <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/c
...rding to a different strategy. Additionally, the protein order within each
construct was randomized to ensure the issue was not caused by specific proteins. In
14 KB (1,769 words) - 02:10, 2 November 2017
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...een populations in real ecosystems, we use methods of synthetic biology to
construct a more simple system using <i>E.coli</i>. The system involves two groups of
...<div class="myPara"> Therefore, our main task is to
construct three characters of the two groups. <br>
18 KB (2,437 words) - 03:33, 2 November 2017
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...e protein ACA3 (see figure 18). We were able to successfully assemble this construct (see gel electrophoresis), however we did not manage to successfully transf
...f our biobrick with 18bp overlaps for Gibson assembly. However, this large construct required 7 fragments (see figure 20) and we were unsuccessful in our attemp
64 KB (8,040 words) - 10:51, 31 October 2017
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<p>As the maximum activity was observed in pPMMVFLt12
construct, for the present study the strong promoter having the synthetic cis element
<p><b>
Construct Formation:</b></p>
14 KB (1,923 words) - 17:58, 29 October 2017
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...The detailed cloning workflow which led to the finished Evaluation Vector
construct with the pBS1C backbone.</figcaption></figure>
...d it into the pSB1C3 backbone (via EcoRI and PstI digest) and verified the
construct by sequencing. It has been submitted to the parts registry under <a target=
13 KB (2,057 words) - 00:02, 2 November 2017
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construct. Our dCas9 will target the region between the transcription start
a component of the natural CRISPR-system, but it is a synthetic construct
12 KB (1,959 words) - 12:52, 24 October 2017
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...signaling cascade for the biosensor with a CRE-luciferase construct as the reporter.
to construct stable cell lines. Based on these cell lines, we infected different
79 KB (5,538 words) - 08:25, 25 October 2017
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<li>
Construct KD Tree based on the normalized word vectors of the key words in the databa
<p>This is the final step to
construct our Recommendation System , overall we use collaborative filtering strategy
27 KB (3,894 words) - 23:43, 1 November 2017
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...unidirectional terminator (ECK120034435) between them. This construct can be regarded as an
...troduced one or two pairs of attB/P sites on both sides of ECK120034435 to construct
40 KB (3,961 words) - 23:39, 1 November 2017
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...proteins on the dual-expression vector (pETDuet-1, pCDFDuet-1) for further construct other circuit using for detection.</p>
...striction on the use of T3 + sfGFP Endonuclease PstI and EcoRI digested to construct the expressed product. The electrophoresis tank buffer was 0.5 x TBE and th
31 KB (3,572 words) - 04:17, 9 December 2017
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...proteins on the dual-expression vector (pETDuet-1, pCDFDuet-1) for further construct other circuit using for detection.</p>
...striction on the use of T3 + sfGFP Endonuclease PstI and EcoRI digested to construct the expressed product. The electrophoresis tank buffer was 0.5 x TBE and th
27 KB (3,288 words) - 19:29, 1 November 2017
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<div class="page-heading">MESA construct replication</div>
The first few weeks, we tried to replicate the MESA construct using Luciferase-GFP fusion protein as the output signal and dsRED as a tra
125 KB (17,334 words) - 16:58, 27 October 2017
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<li> MG1655 transformed with <i>cysPUWA</i> construct in LB media with varying concentrations of potassium chromate (20-160 uM)
<li> MG1655 transformed with <i>cysPUWA</i> construct in LB media with no potassium chromate </li>
11 KB (1,541 words) - 01:55, 2 November 2017
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...apoprotein. After the first time purification, we refolded the protein to construct the correct structure and then we activated the apo-HRP with hemin to produ
...nce we failed to express GFP and ER-alpha at the same time, we will try to construct the gene containing GFP and ER-alpha and express them together for the purp
19 KB (2,852 words) - 01:27, 2 November 2017
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...ntibiotics. Previous work shows that when colonies containing the reporter construct are grown in LB medium there is very little response to the addition of try
...gem.org/wiki/index.php?title=Part:BBa_K2330002"> BBa_K2330002</a> reporter construct)</p>
9 KB (1,404 words) - 03:44, 2 November 2017
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...FP</em> being inserted into a DVK-AF plasmid. <strong>A:</strong> Displays construct assembly, BsaI:R indicates the
...eft. A, E and F = MoClo fusion sites. <strong>B:</strong> Overview of each construct and DVK-AF once it has been cut by BsaI. <strong>C</strong>: After ligation
7 KB (1,210 words) - 01:03, 2 November 2017
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<div class="note-p">Initially we planned to
construct pBBR1-<i>rhlABC</i>-<i>fimS</i>-<i>lacI</i>-<i>lacO</i>-<i>BcsA</i> and pBa
<div class="note-p">We continued OE PCR, simultaneously, we went on to
construct pBad30-cm-<i>fimE</i>.</div>
17 KB (2,496 words) - 03:52, 2 November 2017
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All plasmids except pSB1C3-PtNTT2 were tagged with the same cMyc-GFP construct for imaging and Western Blots. cMyc was used as a linker to allow the targe
...-cMyc-GFP with a TAT signal peptide. No bands can be observed for the full construct, but a very weak band can be seen between 55 and 70 kDa for the fusion prot
69 KB (10,514 words) - 03:26, 2 November 2017
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After obtaining the E.coli, we designed a construct, known as a biobrick, to allow the bacteria to express the proteins lipase
...came the gene for plastic degradation. One construct had esterase, and one construct had lipase.
7 KB (1,019 words) - 19:41, 19 November 2017
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...tical-align:middle;">Cloned <i>rdhANP</i> into PSB1C3 and transformed the construct into DH5α</td></tr>
...tical-align:middle;">Cloned <i>rdhANP</i> into PSB1C3 and transformed the construct into DH5α</td></tr>
31 KB (4,040 words) - 07:51, 1 November 2017
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...giving it yellow color. Construct 1 is cloned at AatII and ApaI site, and construct 2 is cloned at SalI and BamHI site.</h3></section>
...e copy plasmid, pACYC177. Construct 1 was cloned at XhoI and XmaI site and construct 2 was cloned at BamHI and AatII site.</h3></section><br />
5 KB (751 words) - 04:13, 14 December 2017
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<li> pSB1C3-TraR construct from the 11 of September 2017 </li>
<li> pSB1C3-TraAC construct from the 11 of September 2017 </li>
37 KB (5,520 words) - 03:50, 2 November 2017
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...org/Team:UT-Knoxville/Project#PuPromoter">here</a> to read more about this construct. </li>
...uct, our Pu promoter showed ~4x GFP expression than our solely 0.1 mM IPTG construct. Thus, our regulatory sequence including xylR regulatory protein and Pu pr
23 KB (2,783 words) - 02:29, 2 November 2017
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...onstruct 1, Ptet- RBS1- T7 RNA polymerase –terminator 1-terminator 2 and construct 2, PT7-RBS2- Chromoprotein II- RBS3-tetR-terminator3. Different parts like
<h3>For cloning of construct 1 of module 2, Pmar-RBS4- ToxR-terminator 4 and construct 2, Pctx-RBS5- chromoprotein I- RBS6-TetR- terminator5. Different parts like
7 KB (1,022 words) - 04:12, 14 December 2017
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...rescence microscopy images of Low and High strength Anderson promoter-PduD construct associated mCherry expressed alone and co-expressed with Eut subunits.</cen
An added benefit of our SMNLK+PPK construct is that it would allow us to determine whether it actually worked via DAPI
8 KB (1,131 words) - 03:26, 2 November 2017
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<h2>Whole construct assembly design</h2>
Final construct looks like this (Figure 3):</p><br>
15 KB (1,912 words) - 02:32, 2 November 2017
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...>BBa_K2350020</a></b>). Not only this, we even grouped endolysin-holin’s
construct with Nrt-A and created a new part (<b><a href="http://parts.igem.org/Part:B
...xt-decoration:none;">BBa_I742157</a></b>) . Same with indigoidine, we also
construct this part on our special designed backbone pPIGBACK so that it can be expre
6 KB (931 words) - 19:49, 1 November 2017
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...spoke with Team Tokyo Tech, who inspired us to <b style="color:#ff7575;">construct a plasmid containing Melanin (black pigment) sequence</b> for maximizing li
...onths of research on increasing the oil production in algae, we started to construct plasmids which would be used in our project. However, some question arose d
16 KB (2,075 words) - 23:43, 1 November 2017
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.... Suddenly I came up with an interesting idea that what if the scaffold is
construct by <font color="#1E90FF"><b>protain</b></font>. Then I realized that scaffo
<p class="dummy-text">So now how do we
construct the scaffold. We knew that there was many protein self-assembly system but
23 KB (3,147 words) - 09:16, 29 August 2017
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...ctive protein degradation tag (and a pdt-less inducible mScarlet-I control construct), all under the control of the pTet promoter. These parts, in combination w
...he strong constitutive promoter J23100. We also included a tagless control construct (J23100 mScarlet with no pdt) as a comparison. In order to demonstrate that
30 KB (4,615 words) - 02:13, 2 November 2017
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...t molecules can be recruited. This way, it will be possible to enhance the construct with bioactive functionalities. </br>
4 KB (555 words) - 19:55, 27 June 2017
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<p id="homeP">These construct backbones are assembled via Gibson Assembly and transformed into <i>E. coli
...tios to a single tube and assemble the insert and vector into an a plasmid construct.
10 KB (1,326 words) - 03:47, 2 November 2017
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...<div class="figure-text"><strong>Figure 1. The STAR-chromoprotein
construct.</strong></div>
...<div class="figure-text"><strong>Figure 2. The STAR 3 - As - CP
construct</strong></div>
17 KB (1,418 words) - 09:26, 10 December 2017
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The full genetic construct is comprised of three parts: pλR LacI, one of the three tsPurple parts (ts
...G, the relative levels of degradation would be compared; theoretically the construct with no degradation tag would show no degradation, the DAS tag (which is a
11 KB (1,456 words) - 16:09, 16 December 2017
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...y xylulose present, giving the xylulose molecules a chance to activate our construct.
...osities of fluids would cause non-uniform mixing of the samples inside the construct.
7 KB (1,150 words) - 23:05, 1 November 2017
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<figcaption><strong>Figure 9.1:</strong> Sketch of our GFP-LOV-PTS1 construct</figcaption>
<figcaption><strong>Figure 9.2:</strong> Sketch of our PTS2-import construct</figcaption>
85 KB (11,201 words) - 02:57, 2 November 2017
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...In this work, our goal is to construct a biological odor sensor in an easily cultured eukaryotic cells (HEK293) by
...dor compound/receptor pairs (β-citronellol/OR1A1 and bourgeonal/OR1D2) to construct the iSmeller odor sensors and evaluate their sensitivity and specificity. S
22 KB (1,602 words) - 16:07, 25 October 2017
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...ttom of the tube. 10μl of MilliQ ultrapure water was added to each of the construct wells. The reconstituted plasmid DNA was stored at -4°C.</p><br>
...each construct (16 in total). Using P200 pipette tips, 2 colonies for each construct were picked from their respective plates. The test tubes were placed on a l
16 KB (2,686 words) - 03:06, 2 November 2017
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total of 9 total parts that needed to be stitched into a full construct. This led to the use of the Gibson Assembly
in order to increase the rate of success of creating our construct. </h4><br>
13 KB (1,605 words) - 02:04, 2 November 2017
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...que that is similar to blue/white screening. User can use this biobrick to construct their toehold switches that use pT7 as the promoter and mRFP as the reporte
20 KB (2,806 words) - 20:57, 1 November 2017
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...od of time before undergoing knock out by Cre recombinase. Knocking out of
construct will lead to inactivation of the gene of interest, providing a secure and s
<p>There are two scenarios of activity in Time Module
Construct:</p>
4 KB (681 words) - 05:02, 3 October 2017
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...we interviewed different experts to evaluate the safety assessment of our construct. The talks focused on the importance of incorporating deficiencies. After m
...ion that there are no major security vulnerabilities, since we planned our construct according to
6 KB (822 words) - 03:45, 2 November 2017
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<p> The strategy we chose to construct the vector is to fuse B0015 and AmpR together first.
...p>10, 11</sup> Thus, we used IDT service to synthesize these two genes and construct it on pPIGBACK, a vector which can express the carrying genes in <i>S. elon
33 KB (4,501 words) - 17:42, 1 November 2017
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...tion tent which acts to increase the humidity to promote fast germination. Construct with clear plastic and kebab sticks.
...ormed the second gateway reaction with pB2GW7 to produce our final plasmid construct. </p>
39 KB (5,765 words) - 03:46, 1 November 2017
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4. Construct the INP-mSA part by Gibson assembly. <br/>
3. Construct the BirA-pYC230 circuit by T4 DNA ligase and transform into EBY100 strain.
34 KB (3,489 words) - 13:59, 18 November 2017
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...<th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Construct</th>
...ckground fluorescence for both biological replicates. The positive control construct exhibited reproducible patterns of fluorescent protein expression over the
10 KB (1,509 words) - 02:40, 2 November 2017
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<h2>General Protein Construct</h2>
<h2>General RNA Construct</h2>
12 KB (1,662 words) - 02:15, 2 November 2017
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...he usage of our non-canonical amino acids 2-nitrophenylalanine we aimed to construct a purification column that can induce the elution of the target protein by
...oncept. This led to another version of the column we have not been able to construct yet. However, we instead created a 3D-model to show it.
104 KB (12,792 words) - 20:31, 14 December 2017
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...arts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404013"> 35S:Luc+:NosT construct </a>, and quantified the activity of the 35S promoter using a luciferase re
...ref="http://parts.igem.org/Part:BBa_K2404013:Experience">35S:Luc+:NosT</a> construct. The red regions show high levels of luciferase expression. This part can n
6 KB (922 words) - 17:02, 31 October 2017
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...to use a flow cytometer to thoroughly quantify the expression of the deGFP construct, using fluorescence-activated cell sorting (FACS) as this method was not ac
After the arrival of the DNA, we transformed the deGFP construct into <i>E. coli</i> Top10 cells before we ran the sample through the flow c
4 KB (614 words) - 03:32, 2 November 2017
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...rescent Protein (GFP) variant for the first time. We created an expression construct (J23100 promoter and B0034 RBS in front of deGFP) and characterised the deG
...the sarcosine oxidase enzyme for the first time. We created an expression construct (T7 promoter and B0034 RBS in front of SOx CDS) and demonstrated that SOx i
15 KB (2,035 words) - 18:30, 1 November 2017
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The Cre-Cas9 construct was successfully assembled together into a plasmid. To make the P<sub><i>FU
...switch worked. The results came back negative. Sequencing of the Cre-Cas9 construct showed mutations in the Cre-recombinase part and might be the reason to why
256 KB (35,572 words) - 17:24, 30 October 2017
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...from Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria, in the journal Sensors. The backbone, kn
...from Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria. It shows that the promoter expressed GFP
7 KB (1,112 words) - 21:21, 13 October 2017
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...ruct was inserted into the vectors via Gibson Assembly method. The ligated construct was then transformed in competent E.coli and plated on chloramphenicol (CAM
24 KB (3,933 words) - 12:37, 16 October 2017
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...sensor Construct for Use in Gram-Negative Bacteria" published in Cell. The construct was used with permission by Dr. Ashley Franks.
7 KB (1,209 words) - 16:28, 28 October 2017
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<td>Construct comprised of PtNTT2, lacO and Cas9</td>
<td>Construct for the integration of unnatural base pairs</td>
28 KB (4,174 words) - 00:37, 2 November 2017
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...genes hpaB and hpaC in E. coli. To simplify our assembly, we designed our construct to position hpaB and hpaC coding sequences in succession under the control
...vector containing a pMMB67EH ori and spectinomycin resistance marker. Our construct was assembled using golden gate cloning techniques. To do so, we first used
5 KB (681 words) - 05:01, 16 December 2017
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...of the pockets. We choose to start with only empty pockets of the Scaffold Construct.<br/>
<li><span>The binding of an inducer to the pocket of the Scaffold Construct.</span></li>
7 KB (1,169 words) - 22:19, 1 November 2017
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...Ba_J23117" style="color:#C1D35D">j23117</a>. Along with one promoter, each
construct also had one of the two RBS (
...parts.igem.org/Part:BBa_J364100" style="color:#C1D35D">j364100</a>). Every
construct had the same GFP (<a href="http://parts.igem.org/Part:BBa_E0040" style="col
19 KB (2,490 words) - 02:59, 2 November 2017
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...m.org/Part:BBa_K2475002">PrD-YFP</a>, and C is used as a shorthand for our
construct <a href="http://parts.igem.org/Part:BBa_K2475001">PrD-CFP</a>. The samples
<p>In order to determine if [psi-] C was fluorescing because of our
construct, we checked its fluorescence value after 12 hours of growth in YPD. A signi
11 KB (1,552 words) - 03:28, 2 November 2017
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In the near future we would like to finish our final construct for the dCas9 project and measure the Auxin concentration to determine if w
...re how much (ideally: functional) respective protein is produced with each construct.
7 KB (894 words) - 11:24, 1 November 2017
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Our construct
.../. Gene sequence was optimized to eliminate illegal restriction sites. The construct was synthezied by IDT.
3 KB (495 words) - 22:32, 14 December 2017
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<br><br> To have a construct work in <i>Agrobacterium</i> we needed to use a plasmid that could replicat
<i>ietAS</i> construct. The resulting ligation was then transformed into chemically competent <i>E
17 KB (2,525 words) - 03:58, 2 November 2017
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...he plcA gene is under the control of PlcR, its promoter is usually used to construct reporter system.</p>
...take the pDG1730-pAim-AimR-AimP-pAimX-GFP (abbreviated as pDG1730-Aim-GFP) construct as an example. The Aim sequence was synthesized. After that, we cloned the
20 KB (2,735 words) - 13:46, 1 November 2017
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...<b>Figure 3-12 CsgD and OmpR234 Expression </b> Our construct includes a strong promoter, two strong RBS, csgD, ompR234 and double termin
...gure 3-13). Sequencing results from Tri-I Biotech confirmed that our final construct is correct.
25 KB (2,679 words) - 13:33, 31 October 2017
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...sed our engineered strain pMC( strain co-expressed Mtr CAB and Ccm A-H) to construct a bio-cathode. We monitored the current of the bio-cathode to see whether t
...se the efficiency of electron transferring. Then what we need to do was to construct this bio-cathode, put every part of this “toy” together and get the oxy
36 KB (5,138 words) - 15:42, 27 October 2017
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Construct design:
...iosensor were stalled at the first hurdle. Like the erythromycin biosensor construct, both the MDH and FrmR repressed system were successfully inserted into the
5 KB (700 words) - 04:01, 2 November 2017
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Because of the lack of an AafII restriction site in the G1.1 construct there are only two bands to be seen. <br/>
<em class="u">SEAP construct (19, 20)</em> are send to sequencing.
147 KB (23,004 words) - 22:13, 31 October 2017
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...led two plasmids that allow for light-sensitive gene expression. The pDusk construct allows for transcription in the absence of light.
...acteria to survive in unprecedented high-stress laboratory conditions. Our construct will also be foundational to modifying plant and other eukaryotic cells to
28 KB (3,299 words) - 04:18, 31 October 2017
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...ic machine and biologic response with medium wave. We desperately tried to construct such a interface.</p>
...ating sequence(UAS)of CYC1 promoter with 4 CDREs. It can be a hard work to construct such a promoter with these repeating sequences. Luckily, UCAS helped us to
33 KB (3,975 words) - 16:41, 1 November 2017
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<p class="capture">Fig.2 Pko1 with deletion construct</p>
...ity of the whole construct. Therefore, the remaining work is to insert the construct into the vector pko1 and transformation of this plasmid. And we will try to
22 KB (2,625 words) - 16:08, 1 November 2017
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<figcaption>Figure 1: The organization of our <em>NahR</em> construct.</figcaption>
<figcaption>Figure 2: The organization of our <em>GFP1-9</em> construct, transactivated by <em>NahR</em>.</figcaption>
6 KB (783 words) - 01:45, 2 November 2017
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them to construct hpRNA producing vector ecrL, which sped up their vectors construction
<h3>Teammates of Lanzhou helped us to construct the distribution of the olfactory receptor
27 KB (2,368 words) - 00:54, 2 November 2017
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...a different organism that would be better suited to shuttling our genetic construct for a longer period of time under a wider range of conditions.
...e to show a colour change, much like we were aiming to do with our genetic construct.
9 KB (1,371 words) - 17:27, 1 November 2017
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...you want. This function is designed to help specify your need, to help you construct your idea better.
...e of simulation is the uncertainty of genetic circuits since the users can construct them arbitrarily. Therefore we built a rather generic model capable of simu
50 KB (6,406 words) - 01:44, 2 November 2017
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...you want. This function is designed to help specify your need, to help you construct your idea better.
...e of simulation is the uncertainty of genetic circuits since the users can construct them arbitrarily. Therefore we built a rather generic model capable of simu
55 KB (7,186 words) - 05:37, 16 December 2017
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Successfully created pSB1C3: mRFP1 + CpxP construct using GoldenGate.
Repeat of pSB3T5: pBAD + MalE31 construct, again without success.
8 KB (1,318 words) - 13:02, 31 October 2017
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...ave non-complimentary non-palindromic ends. Due to this, the pieces of our
construct would anneal in the orientation that we desired: LacIq, PcrA, and PcrA/B.<p
...in chlorite. The contrast in toxicity can be used to determine whether our
construct was successfully incorporated into our plasmid. We would do this by plating
3 KB (468 words) - 00:36, 21 November 2017
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...FP for prokaryotic expression, and of course, tried to pick up the gene to construct the plasmid with smURFP.
...ng the HU-meaningless-smURFP to the PGH-PMB1. In addition, we successfully construct the co-expression vector.
71 KB (6,381 words) - 19:00, 1 November 2017
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...nce for project in bacteria. In synthetic biology, one central route is to construct a controllable biological network, and electing suitable transcription fact
...TF) - synthetic promoter (SynPro) pairs. This set is a powerful toolbox to construct customized and orthogonal transcriptional network.
9 KB (1,354 words) - 23:47, 1 November 2017
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...<div class="figure-text"><strong>Figure 1. The STAR
construct. </strong>In the absence of antisense, sense target RNA will form a stem-lo
...<div class="figure-text"><strong>Figure 5. Characterization of STAR 3
construct. </strong>In the absence of Antisense 3, little sfGFP will be expressed wit
27 KB (3,221 words) - 03:00, 2 November 2017
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...ar-domain and 229 amino acids to the C-terminal EnvZ-domain. The assembled
construct is 1516 base pairs long and registered as <i>BBa_C0082</i>. from the iGEM T
...sured after a mDAP induction of cells that were transformed with the whole
construct. The results were published <a href="https://2016.igem.org/Team:Hamburg/Dem
17 KB (2,569 words) - 22:21, 1 November 2017
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...istance to carbenicillin in a dose-dependent manner in XL1-blue, while the construct does not seem to work as good in TolC.
The GyrBR_EC construct shows a reduced zone of inhibition in TolC. The small zone of inhibition in
15 KB (1,754 words) - 12:45, 12 December 2017
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...<div class="figure-text"><strong>Figure 1. The STAR-chromoprotein
construct.</strong></div>
...<div class="figure-text"><strong>Figure 2. The STAR 3 - As - CP
construct</strong></div>
17 KB (1,537 words) - 12:43, 9 December 2017
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...nthesize acrylic acid based on glycerol metabolic pathway in organisms and construct a cell factory with a high yield of acrylic acid.
...ce for GLY DH through the two layers of substrate level cycle. At last, we construct
22 KB (2,324 words) - 19:35, 1 November 2017
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...he network that could be targeted practically to improve efficiency of the construct (Figure 1). We used TinkerCell software for this purpose.
...sulfate permease. Here we want to see how sulfate affect the output of our construct. In experimental part we were using experimental concentration of sulfate a
27 KB (3,589 words) - 02:43, 2 November 2017
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...PgntK</i> promoter+mCherry construct versus a <i>Pcon</i> promoter+mCherry construct. The data indicates that <i>PgntK</i> promoter has a comparable transcripti
...PTG induction. In a future test, we would substitute <i>mCherry</i> in our construct for <i>BamHI</i> and measure OD<sub>600</sub> over time in cultures with ch
9 KB (1,214 words) - 15:31, 16 December 2017
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...<div class="figure-text"><strong>Figure 1. The STAR
construct. </strong>In the absence of antisense, sense target RNA will form a stem-lo
...<div class="figure-text"><strong>Figure 5. Characterization of STAR 3
construct. </strong>In the absence of Antisense 3, little sfGFP will be expressed wit
27 KB (1,937 words) - 15:45, 15 December 2017
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...rescent Protein (GFP) variant for the first time. We created an expression construct (J23100 promoter and B0034 RBS in front of deGFP) and characterised the deG
...the sarcosine oxidase enzyme for the first time. We created an expression construct (T7 promoter and B0034 RBS in front of SOx CDS) and demonstrated that SOx i
15 KB (2,059 words) - 20:48, 28 October 2017
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...appropriate. This is because the in vivo lab work was to test whether our
construct worked, rather than finding a relationship between iron (II) ions and amilC
<p>(2) Prove that our
construct would or would not work in an in vitro environment in a cell-free system.</
49 KB (7,597 words) - 23:23, 14 December 2017
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<h1>The Scaffold Construct: 14-3-3</a>
<figcaption>Figure 1: Construct consisting of 14-3-3 monomers and GFP</figcaption></div> <br/> </h6>
14 KB (2,113 words) - 13:46, 10 December 2017
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the whole construct as shown in Figure 5 below. This design is used for the detection of the ex
the peptides from the whole construct. It is very likely that if the peptide LL-37 plus the histidine tag
27 KB (2,825 words) - 04:13, 27 November 2017
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...fluorescence system is described by the ordinary differential equation to construct the model. In the second part, we tentatively designed a single fluorescenc
...and had a great amplification effect. During the next experiment, we could construct a feasible dual fluorescence detection system based on the literature.</p>
21 KB (3,053 words) - 03:03, 2 November 2017
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...led two plasmids that allow for light-sensitive gene expression. The pDusk construct allows for transcription in the absence of light.
...acteria to survive in unprecedented high-stress laboratory conditions. Our construct will also be foundational to modifying plant and other eukaryotic cells to
29 KB (3,358 words) - 17:44, 1 November 2017
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<h3> Verifying the construct </h3>
...t, we expect that levels of red output will rise with increase in reporter construct. These expected results can be seen below. The different colored lines corr
5 KB (893 words) - 01:33, 2 November 2017
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<h3> Verifying the construct </h3>
...expect that levels of blue output will rise with the increase in reporter construct, because YFP will be skipped over, allowing BFP to be produced. These expec
5 KB (825 words) - 23:03, 1 November 2017
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In our final construct, FrmR is placed under the control of promoterfrmR, and Mecherry rfp is pla
...f mRNA molecules to get the total translation rate per second of each DNA construct.</p>
22 KB (2,959 words) - 13:50, 1 November 2017
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...ours and diluting them to an OD600 of .005, we simultaneously induced each construct with 0.1mM IPTG and 50ng/mL ATC. Simultaneous induction was chosen because
...olor: #808080; font-size: 12px;'>Figure 1: Schematic of a generic reporter construct used to test gene expression speed. mScarlet-I is produced in the prescence
7 KB (1,046 words) - 03:34, 2 November 2017
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...me to conduct transformation of yeast. In this competition, all teams must
construct plasmids which include pSB1C3 backbone. However since this backbone does no
<p>To
construct plasmid 3, we amplified 3 terminators from <i>Saccharomyces cerevisiae</i>
30 KB (4,800 words) - 03:24, 2 November 2017
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...or, which serves as both, a mPTS and transmembrane domain. We designed our
construct with mRuby2 fused to the N-terminal side of the <a href="http://www.uniprot
...<a href="http://www.uniprot.org/uniprot/P28795">Pex3</a> (AA 1-39). This
construct should be N-terminally anchored in the peroxisomal membrane, with mRuby2 ag
15 KB (2,389 words) - 00:58, 2 November 2017
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...fluorescence system is described by the ordinary differential equation to construct the model. In the second part, we tentatively designed a single fluorescenc
...and had a great amplification effect. During the next experiment, we could construct a feasible dual fluorescence detection system based on the literature.</p>
19 KB (2,915 words) - 18:12, 31 October 2017
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...ding sequences, so that it can be amplified similarly to our first His-TEV construct, but the amplicon is only 194 bp. This was a great benchmarking sequence, a
...ing phenol-chloroform extraction. As our initial amplicon from the His-TEV construct was too long (1262 bp compared to 500 bp optimal amplification length from
18 KB (2,911 words) - 03:59, 2 November 2017
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...w 34C for a prolonged length of time. As proof of concept, we attempted to construct the kill switch in <i>E. coli</i>. We designed experiments to test the rate
Although we developed our construct in E. coli as a proof of concept, we envision the final product being incor
9 KB (1,427 words) - 01:04, 2 November 2017
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<p>Until the Wiki freeze, we tried to
construct plasmid with the insert we designed and managed to combine plasmid with INS
<p>To assay our
construct, we cultured E. coli with IPTG induction, purified the crude extract by the
3 KB (504 words) - 03:57, 2 November 2017
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...s produced, the promoter driving expression of the GFP is 'cut' out of the
construct and flipped, such that the promoter is now on the opposite strand. This mea
...when grown on 0.5% w/v glucose) is still too active. The design for this
construct has been submitted (<a href="http://parts.igem.org/Part:BBa_K2205006">BBa_K
15 KB (2,234 words) - 21:53, 1 November 2017
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Eppendorf 1: Rsal 1 construct cut with Ecor1 + PST1
Eppendorf 2: Rsal 2 construct cut with NOT1
5 KB (680 words) - 19:06, 1 November 2017
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...se (CrtZ). To make Synechococcus elongatus PCC 7942 produce zeaxanthin, we construct a plasmid BBa_K2320022 under the control of PrbcL. After the expression of
...CO2 bio-mitigation by genetic engineering of cyanobacteria) and decided to construct pigment plasmid with the same promoter. The natural ribosome binding site i
5 KB (761 words) - 15:56, 1 November 2017
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...ze:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Bioengineered construct</center>
...nt is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
3 KB (447 words) - 23:02, 1 November 2017
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...39007</a>). Furthermore, the native CpxP gene was assembled into a similar
construct, to act as a control (<a href="http://parts.igem.org/Part:BBa_K2387014">BBa
...hermore, the MalE24-1-CpxP fusion was also created and assembled into this
construct, to act as a control (<a href="http://parts.igem.org/Part:BBa_K2387022">BBa
28 KB (3,662 words) - 15:50, 6 December 2017
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...th and recommended ATCC broth for <i>Pc. denitrificans</i>. The goal is to construct a growth curve of the relatively uncharacterized chassis for use in future
...ing composite biobricks by using restriction digests on the prefix of this construct. Digested promoter sequences for VhB, Mn-SOD, and a T7 constitutive promote
29 KB (4,173 words) - 03:55, 2 November 2017
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...<h3>In this part, we validate how ceaS2 works as expected and how we construct GAACF1.0.</h3>
enzyme directly into the chassis cells, we can construct a new pathway of acrylic biosynthesis based
19 KB (2,150 words) - 20:58, 1 November 2017
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<li>Balthasar Schlotmann <span class="info-text">- IT, Construct design</span></li>
<li>Kenneth Wolters <span class="info-text">- Construct design</span></li>
4 KB (535 words) - 06:03, 22 November 2017
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...rent trapping abilities of our PR construct (BBa_K2229400) and our biofilm construct (BBa_K2229300). <b>After experimentally determining the rate constants for
76 KB (8,634 words) - 14:19, 1 November 2017
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...rom DAPI stained E. coli expressing Medium strength Anderson promoter-PduD construct.</center></p>
...bution of the tag. This suggested that, if working properly, images of our construct would display clear accumulation of both mCherry and DAPI signals in the sa
6 KB (804 words) - 02:26, 2 November 2017
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...standards. To address this we have removed all illegal cut sites from the construct.
...As such, we expressed and purified MS2BP along with our tRNA<sup>Phe</sup> construct. From this we concluded that MS2BP expresses, binds to Nickel Sepharose aff
8 KB (1,233 words) - 03:02, 2 November 2017
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.../i> construct could be replaced with a <i>P<sub>hrpL</sub>-rbs30-sfGFP</i> construct), the individual parts are still coupled tightly together on the same DNA m
22 KB (3,161 words) - 19:00, 1 November 2017
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...for the full sequence of mRFP, a red fluorescent protein, above 37°C. The construct is composed of a cI857 coding gene behind the strong constitutive promoter
...above 30°C until 42°C, when it can no longer bind to pL. The aim of this construct is to code for mRFP when the temperature rises above 30°C, while the trans
25 KB (3,703 words) - 03:42, 2 November 2017
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...us via other non-commercial sources. Thus, as a side project we decided to
construct a <a href="https://2017.igem.org/Team:BOKU-Vienna/Experiments#CRISPR">plug
27 KB (3,932 words) - 16:27, 1 November 2017
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<li class="my-content-li2">2.
Construct a response signal circuit to confirm that our receptors can detect the swee
<p class="my-content-p">We used the synthetic genes T1R2, T1R3 to
construct the gene circuits for receptors expression. The galactose-induced promoter,
25 KB (3,422 words) - 11:24, 31 October 2017
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The aim of our project is to construct a learning platform in <i>C.elegans</i>. This platform should be able to pr
We need to construct a system inside the worms which can make them show specific response to the
30 KB (3,996 words) - 16:46, 14 December 2017
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<div id="apDiv10">1. Construct our warriors and beggars by co-transformation. (the gene circuits are as fo
5.Construct the following 6 groups and then incubate the cultures at 37℃,shaking at 2
16 KB (2,205 words) - 12:48, 25 October 2017
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...the Anza enzyme system protocol. See agarose gel below for confirmation of construct.</p>
5 KB (791 words) - 02:48, 2 November 2017
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<p>Digestion of double E3/Repressor
construct to show presence of GFP/Expressor in gel</p>
<p>Write protocol to begin
construct tests with AFB1</p>
6 KB (803 words) - 01:10, 30 October 2017
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<h3>Our
Construct-<a href="http://parts.igem.org/Part:BBa_K2260002">Part: BBa_K2260002</a></h
...em.org/mediawiki/2017/3/32/Calgary2017_PhasinConstruct.png" alt="Secretion
Construct" style="width:100%"></center></p>
15 KB (2,525 words) - 02:41, 2 November 2017
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...enetic parts are produced commercially. We use homologous recombination to
construct our entire network.</li>
<p>Despite using dCas9 to
construct a NOR gate, no gene drive is created. By following the due process, we were
10 KB (1,542 words) - 23:15, 31 October 2017
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...<td>OR1A1 expression vector is transfected into HEK293FT cells to construct the signaling circuit in response to odor molecule β-citronellol.</td>
...<td>OR1D2 expression vector is transfected into HEK293FT cells to construct the signaling circuit in response to odor molecule bourgeonal.</td>
32 KB (2,231 words) - 16:23, 25 October 2017
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...project, there was not sufficient time to completely redesign our genetic construct such that it could be expressed in <i>Bacillus subtilis</i>. Because of thi
17 KB (2,545 words) - 00:46, 2 November 2017
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...ll the necessary cellular components in cell-free extracts and our genetic
construct which are embedded into the paper and freeze-dried. They are easily stored
<p>In order to react to different iron levels, our
construct will be promoted by the AceB gene, which can be regulated by FUR, a transcr
48 KB (6,039 words) - 22:09, 14 December 2017
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...ll the necessary cellular components in cell-free extracts and our genetic construct which are embedded into the paper and freeze dried. They are easily stored
In order to react to different iron levels our construct will be promoted by the AceB gene, which is usually involved in carbon sour
2 KB (350 words) - 19:09, 29 June 2017
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...at which denatures our DNA, which would undermine any synthetic biological construct. Due to the fundamentally simpler nature of our circuit and resultant cost
...-color: rgb(255, 255, 255);">The electrical signal produced by a bacterial construct would be small, requiring an amplifier to increase the signal for interpret
55 KB (6,136 words) - 02:31, 1 November 2017
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to construct stable cell lines. Based on these cell lines, we infected different
virus to construct a super cell line, which has a high expression of the three genes.
29 KB (2,130 words) - 09:42, 30 October 2017
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<h3> Construct Building and Validation </h3>
As a first quick test of our construct, we transformed pT8-O and a plasmid not containing adenylate cyclase (as a
6 KB (863 words) - 03:37, 2 November 2017
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<p>Our project is to construct a three-enzyme system to degrade environmental pollutant 1,2,3-trichloropro
...of the 1,2,3-TCP pathway in the last studies[2]. So we chose the DhaA31 to construct plasmid. We then evaluated the effects of enzyme stoichiometry on efficienc
26 KB (3,639 words) - 03:15, 2 November 2017
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...anscription factors and promoters were only predicted, we had to build the construct to test their behavior. We talked about our plans with Dr. Fournier, from
...parts.igem.org/Part:BBa_K2448024" target="__blank">BBa_K2448024</a>). This construct aims to provide an answer to the lack of rapid and reliable building metho
30 KB (4,436 words) - 18:31, 15 December 2017
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...ts that we sent them degraded as expected. The difference in the remaining construct could be due to differences in growth conditions (LB vs. M9 media), plate r
10 KB (1,542 words) - 03:47, 2 November 2017
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<div class="col-p">Besides, Team Tianjin helped us
construct a plasmid that can express lyase upon the presence of xylose.</div>
<div class="col-p">We helped NEU-China
construct the distribution of the olfactory receptor 1D2 with a Rho tag in the N-term
6 KB (856 words) - 02:11, 2 November 2017
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...sed our engineered strain pMC( strain co-expressed Mtr CAB and Ccm A-H) to construct a bio-cathode. We monitored the current of the bio-cathode to see whether t
...se the efficiency of electron transferring. Then what we need to do was to construct this bio-cathode, put every part of this “toy” together and get the oxy
27 KB (3,414 words) - 14:49, 1 November 2017
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...p><p dir="ltr">Validate the use of LC 480 II utilizing T7 constitutive GFP
construct- Successful</p><br><p dir="ltr"><span id="docs-internal-guid-2e42da7c-1809-
79 KB (8,459 words) - 15:31, 1 November 2017
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...izer for the interaction between the scaffolding construct and the binding construct. Research has shown that fusicoccin in a concentration 12 times larger than
7 KB (1,092 words) - 16:44, 1 November 2017
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To
construct the sgRNAs, primer dimers were PCR amplified to generate the initial fragme
<p>To
construct the sgRNAs, primer dimers were PCR amplified to generate the initial fragme
31 KB (4,631 words) - 03:59, 2 November 2017
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...sothermal reaction using unpurified enzyme worked, the allegedly assembled construct must be transformed into DH5α cells. The DNA can then be purified from the
...sis organism was e. Coli. We did not work with other organisms to test our construct. We used E. coli strains BL21 and DH5-α.</p>
17 KB (2,315 words) - 04:00, 2 November 2017
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...pVEG, has a constitutive ranscription rate of 3.00PoPS per molecule of DNA construct, and this can be translated to </p>
17 KB (2,149 words) - 03:59, 2 November 2017
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...ing the test promoter construct and cells containing the standard promoter construct, is negligible compared to the maturation rate of GFP. <br><br><hr><br>
15 KB (2,092 words) - 02:47, 31 October 2017
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...th reactions set up as per manufacturer protocol, with replicates for each construct. PCR programmes varied but typically utilised a touchdown protocol which im
...products analysed using agarose gel electrophoresis to confirm if correct construct was present. </li>
67 KB (6,501 words) - 19:32, 27 July 2017
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...t:16px;">Because organisms do not naturally synthesize tagatose, we had to construct the metabolic pathway for this reaction to occur. We used a number of sourc
...style="margin-left:16px;">After we were done with each individual BioBrick construct and validated the enzymes via DNA sequencing and confirmation, we then deci
14 KB (2,058 words) - 02:54, 2 November 2017
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...th reactions set up as per manufacturer protocol, with replicates for each construct. PCR programmes varied but typically utilised a touchdown protocol which im
...products analysed using agarose gel electrophoresis to confirm if correct construct was present. </li>
67 KB (6,513 words) - 15:21, 31 July 2017
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...ing the test promoter construct and cells containing the standard promoter construct, is negligible compared to the maturation rate of GFP. <br><br><hr><br>
14 KB (2,051 words) - 23:11, 17 October 2017
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...ing the test promoter construct and cells containing the standard promoter construct, is negligible compared to the maturation rate of GFP. <br><br><hr><br>
10 KB (1,452 words) - 21:41, 18 October 2017
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...<p>We add the gene of P-asr before the gene of glsa and construct a plasmid with pet28 as the backbone. We then transform it into <i>E.coli</
...nus cultured individually when cultured with E.coli at pH=5 ，so we first construct the PPGM-PGM-331 plasmid and verify its effect on BC yield after overexpres
38 KB (4,970 words) - 03:57, 2 November 2017
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<h2> Construct Design</h2>
<li class="text14"><b>Initial Translation Components Construct Compilation (sequence retrieval and codon optimization)</b>: Simmone D'Souz
19 KB (2,788 words) - 03:23, 2 November 2017
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<td data-title="Description">YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)</td>
<td data-title="Description">YddG-AroG construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455000) </td>
8 KB (999 words) - 03:25, 2 November 2017
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<h3 class="subtitle">
Construct 1 (GlmY with a constitutive promoter):<h3>
<p style="text-align: center;">Figure 2 shows a characterized
construct of part number BBa_K1320100. J23102 is a constitutive promoter, while K2221
2 KB (222 words) - 03:52, 2 November 2017
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...ymer production at genetic level, we need to choose a strain with the gene construct that has highest yields.
One way is to choose the gene construct that has the steepest slope of protein expression. However, while the under
13 KB (2,071 words) - 03:52, 2 November 2017
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<p>Each cell received a protein, guide sequence, and reporter construct. Following transfection, some cells received a DOX induction curve to intro
...ok under the tab for specific experiments and results for a given reporter construct.</p>
10 KB (1,525 words) - 02:48, 2 November 2017
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...ng our <a href="https://2017.igem.org/Team:Calgary/Composite_Part">genetic
construct</a>. MATLAB has Cobra toolbox that is used for flux balance analysis and vi
...esis after inserting our <a href="http://parts.igem.org/Part:BBa_K2260000">
construct</a>.</p>
15 KB (2,355 words) - 01:33, 2 November 2017
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...peroxisome without harming the cells. This could be another reason why our construct did not work.
Microscopy of the sample in which this construct was expressed via the strong constitutive promoter ScCCW12 reveals a high n
11 KB (1,728 words) - 03:39, 2 November 2017
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This year, we helped NAU-CHINA to construct two composite bioparts from the plasmid pFA6a-GFP(S65T)-kanMX6 into the pla
...xpress our sincere thanks to NAU-CHINA who made lots of efforts to help us construct our mathematical
15 KB (1,447 words) - 08:37, 25 October 2017
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...visiae BY4742</i> was used for modification. By digestion and ligation, we
construct vika gene on plasmid <i>pRS416</i> which contains a selective marker <i>Ura
<p>In the future, we plan to
construct this biosensor on chromosomes to see whether the result will be more positi
81 KB (11,780 words) - 14:44, 1 November 2017
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a positive GFP control virus and an appropriate blank control viral construct. Leave one well of
virus and an appropriate blank control viral construct. Leave one conical tube as uninfected cells as
14 KB (1,961 words) - 01:42, 2 November 2017
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...enetic parts are produced commercially. We use homologous recombination to
construct our entire network.</li>
<p>Despite using dCas9 to
construct a NOR gate, no gene drive is created. By following the due process, we were
10 KB (1,531 words) - 02:01, 1 November 2017
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...e following constructs into biobricks, but did not succeed with the 3 gene
construct.
We first growed the E.coli, transformed with the single, double and triple
construct vectors described above in <a href="https://static.igem.org/mediawiki/2017/
24 KB (3,423 words) - 00:53, 2 November 2017
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...A at amino acid position 2 of the small subunit are shown in figure 6. The
construct <a href="http://parts.igem.org/Part:BBa_K2201360">BBa_K2201360</a> containi
<th style="width: auto">
Construct name </th>
18 KB (2,887 words) - 02:47, 2 November 2017
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...rent antibiotic resistances. In context of a better while transforming the construct with a low copy plasmids (Wang <i> et. al.</i>, 2001, Wang <i> et.&nbs
For this construct, the incorporation of ncAA is detectable throughout the fluorescence of the
12 KB (1,805 words) - 03:23, 2 November 2017
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...ted us to try and simplify the purification and production of our Winsulin construct in order to reduce the expense associated with insulin, and make it more ac
...Trademark Attorneys, whose feedback we fed into the design of our Winsulin construct so as not to encroach on any existing patents<br /><br /></span></li>
47 KB (6,536 words) - 02:18, 16 December 2017
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...YFP was cloned into the vector using TAR. This lead to the following final construct in the yeast cells, as depicted in figure 2.
Figure 2: Final construct of Ddx4-YFP in pYES2-CT. <br> <br>
11 KB (1,516 words) - 18:20, 1 November 2017
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...onstructs probably are more diffusion limited than they calculated, as our construct are very large proteins and therefore will experience much more resistance.
...stagnates after some time. This is because all the pockets of the scaffold construct are already filled with the inducer. Data supporting the inducer excess is
10 KB (1,607 words) - 21:13, 1 November 2017
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<p>In our project, we wanted to express the whole biosensor construct as a single, composite part. However, that approach has some disadvantages.
...biotinylated at the N-terminus with a chemical reaction. The biotinylated construct can be bound to streptavidin beads, which are widely used in molecular biol
28 KB (3,966 words) - 17:26, 1 November 2017
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...uced modifications at position 1 of the mature FimH protein. We decided to construct a suite of FimH modified sequences to not only test a variety of metal bind
...act as both a reporter of expression but also to bind Ni. Unfortunately no construct was successfully built containing plastocyanin.</p>
8 KB (1,210 words) - 22:31, 1 November 2017
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<p>We have expressed this construct in BL21(DE3). Fluorescence was measured using a plate reader (Tecan) and an
<b>Figure 6:</b> Western blot of BL21 WT and BL21 with our T7-FimH_225_GFP construct, 1-5: sfGFP, BL21 WT soluble fraction, BL21 WT pellet, BL21 with T7-FimH_22
24 KB (3,559 words) - 02:38, 2 November 2017
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...would look like at any distance. We perform this calculation many time to construct a stack of images, each of which represents a cross-section of the 3D space
7 KB (912 words) - 05:00, 30 October 2017
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...nstruct biosensors. This toolbox will contain devices which can be used to construct biosensors. As the toolbox will contain various devices, as well as chassis
2 KB (285 words) - 12:10, 7 September 2017
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...t to create a sequestering mechanism our gold anode. As stated above, each construct was codon optimized for E. coli codons and scanned for illegal sites prior
...this years competition. However, we will continue to work on building this construct because we feel this portion of our project is potentially an important ste
14 KB (2,313 words) - 23:36, 29 October 2017
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...is as it could potentially be a positive result, given that the Nap operon construct does not contain the LacI gene and could therefore be constitutively expres
<i>Fig. 6. Sequence alignment of edited NrfB pSB1C3 construct. H89Y mutation is labelled.</i>
12 KB (1,759 words) - 18:56, 1 November 2017
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<h5>Biobrick construct</h5>
<h5>Lab work, biobrick construct</h5>
21 KB (2,412 words) - 17:05, 31 October 2017
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<a href="#Plasmid-
Construct">Plasmid
Construct</a>
<div id="Plasmid-
Construct">
29 KB (3,813 words) - 15:26, 23 October 2017
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...ped design and codon optimize the parts for the phasin-hemolysin secretion construct. Conducted SDS-PAGE experiments to identify the production of the phasin pr
...ptimized and removed restriction sites from the hemolysin-phasin secretion construct. Cloned in the DNA and performed assays to measure PHB secretion. Completed
16 KB (2,577 words) - 04:40, 16 December 2017
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...png"><span><strong>Fig. 3: </strong><strong>A</strong>) A schematic of the construct used to generate RNA aggregates that contain MS2 aptamers. <strong>B</stron
...: high concentration (full induction) of inducer to no inducer. Strain and construct: BL21AI, FA and FB are under T7lac promoters, and 49xCAG-12xMS2 RNA repeats
12 KB (1,556 words) - 03:40, 2 November 2017
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...expected size of CsgD full construct is 1100 bp (orange box) and OmpR full construct is 1200 bp (blue box).<span class="subCred"> Cloning: Catherine Y., Dylan L
22 KB (2,172 words) - 05:30, 1 November 2017
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<p> This year, we aim to construct several different xylose-inducible expression vectors compatible for <i>B.
...ntaining your target gene, the problem is most likely associated with your construct.</p>
29 KB (4,433 words) - 03:48, 1 November 2017
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...origin; third, our light-inducible system is also of fungal origin. After construct been built, three constructs will be sent into yeast. Our yeast can be stim
...situation by using PyMOL. Consequently, the bio-development group able to construct the plasmid base on this docking result.
11 KB (1,557 words) - 03:05, 16 December 2017
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...d and sent to us. We also encountered issues generating our WRKY:Luc+:NosT construct that we had aimed to send to Valencia, meaning we could not complete the co
4 KB (595 words) - 15:20, 30 October 2017
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...ill try to tune <span class="math">\( K_{\text{Lac}} \)</span> so that the construct behaves differently in healthy and tumor tissue.</p>
...the range <span class="math">\( \SIrange{1}{7.1}{\milli M} \)</span>. For construct <code>K1847009</code>, we know that (ETH 2015) <span class="math">\( K_{\te
21 KB (3,244 words) - 00:48, 15 October 2017
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...experiments and thus directly prove that their experimental setup and our construct function. We organised a video conference, but the plan was rejected, becau
...<h2 id="SVCEChennai" class="section-sub">Support by sending our Promotor Construct</h2>
10 KB (1,513 words) - 03:02, 2 November 2017
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...ich was so nice to offer us a lot of free sequencing labels. Slowly the pH-construct got ready for expression!
Expression of the pH-construct posed some major challenges. First of all, buffering capacity of the M9 med
38 KB (4,669 words) - 03:46, 2 November 2017
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...pET26b(+) expression vector system, under control of the T7 promoter. The construct was then transformed into chemically competent BL21 DE3 <i>E. coli</i> and
A protein around 50 kDa is over-expressed in cells exposed to IPTG with the construct containing beta-xylanase(Fig. 7). Since this protein is not present without
24 KB (3,454 words) - 03:18, 2 November 2017
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...et.We based the human sweetness receptor on our design and hypothesized to construct the signal conduction path in the yeast</p>
...et.We based the human sweetness receptor on our design and hypothesized to construct the signal conduction path in the yeast</p>
8 KB (1,229 words) - 08:31, 5 October 2017
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...et.We based the human sweetness receptor on our design and hypothesized to construct the signal conduction path in the yeast</p>
...et.We based the human sweetness receptor on our design and hypothesized to construct the signal conduction path in the yeast</p>
9 KB (1,281 words) - 10:33, 5 October 2017
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...reening gel, complete construct PCR amplified with screening primers whole construct ~6600 bp
24 KB (2,978 words) - 02:11, 16 December 2017
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We helped NEU-China construct an olfactory receptor Rho-OR1A1. The 64th base pair from the initiation co
So NEU-China students helped us construct a hpRNA producing vector ecrL, which speeded up our vectors construction pr
6 KB (770 words) - 12:01, 1 November 2017
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...ccess, through determining and comparing the results to each corresponding construct’s predicted plasmid length. </p>
...parts. The restriction enzyme that we choose will allow us to separate our construct from the plasmid backbone.</p>
94 KB (10,051 words) - 15:57, 1 November 2017
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...to adapt to the transcription environment in yeast. We did overlap PCR to
construct them together. After that, we sequenced these parts, and sequencing results
<p>In the future, we plan to
construct this biosensor on chromosomes to see whether the result will be more positi
82 KB (11,873 words) - 03:27, 2 November 2017
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...te which prevents network formation. This inhibition site is linked to the construct with a linker which contains a specific protease cleavage site. Proteases e
12 KB (1,891 words) - 16:07, 1 November 2017
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...r biology software that is easier to use than pen and paper. Now every DNA construct made in our lab can be documented in a rich electronic format… and thanks
...up in two parts; one focusing on the purification and one focusing on the construct. Final design is moving towards detecting tetracycline resistance under var
101 KB (13,206 words) - 01:58, 1 November 2017
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...d its terminator. She also gave us some advices for the elaboration of our construct.</p>
...rried out. Finally, we were able to observe the bands, which validated the construct with the part that was sent.</p>
16 KB (2,403 words) - 02:27, 2 November 2017
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<li class="my-content-li2">2.
Construct a response signal circuit to confirm that our receptors can detect the swee
<p class="my-content-p">We used the synthetic genes T1R2, T1R3 to
construct the gene circuits for receptors expression. The galactose-induced promoter,
25 KB (3,436 words) - 01:35, 16 November 2017
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...ormaldehyde which has part number BBa_K1334002. However, when we built our construct from the kit we could not manage to obtain precise induction data. We desig
7 KB (1,169 words) - 01:52, 2 November 2017
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...e plasma membrane and its photo-activated release into the cytoplasm (test construct for BBa_K2332317)</p></td>
...rogrammable transcription factor for reporter/target gene activation (test construct for BBa_K2332317)</p></td>
10 KB (1,234 words) - 23:54, 1 November 2017
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...teins. Considering its strong logical feature, we determine to use STAR to
construct a multi-factor bio-detector. There is a series of STARs which have been rat
<p>In order to
construct a visible detection system, we determine to use chromoprotein as our report
14 KB (1,453 words) - 02:36, 18 November 2017
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...enerators have their illegal sites removed. SCre and VCre are the original construct; SCremut1 is after removing the first illegal PstI site, and SCremut2 are a
...-transformed both of our T7-LacO-recombinase generator and our measurement construct into E. coli BL21 (DE3). These strains are then incubated with or without I
17 KB (2,315 words) - 20:07, 1 November 2017
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...center><font size=2>[ Figure. 1.5-3 ] Snapgene overview of NTHU Taiwan’s
construct.</font></center></p>
<p><br><br>TU Delft offered to revise their SnapGene files featuring their
construct to discard a faulty design as the cause and shared a series of recommendati
22 KB (3,261 words) - 13:20, 1 November 2017
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...r example, we taught them how to construct plasmid and we helped SIAT-SCIE construct their core plasmid in their project.
4 KB (567 words) - 02:11, 15 December 2017
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...stem based on CpxR-CpxR dimerization (Figure 1A) was the most promising to construct in the lab as it shows strong and specific fluorescent signals <i>in silico
...with BiFC is indeed a viable option. Because we put the CpxR-eYFP-termini construct under control of the inducible pBad/araC promoter we were able to test hypo
20 KB (2,298 words) - 22:39, 1 November 2017
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<p>The aim of the following construct is to synthesize polyhydroxyalkanoates from available carbon sources. </p>
...seudomonas putida KT2440 and in most E.coli strains. The first gene in the construct is fabG that turns 3-ketoacyl-ACP into (S)-3-hydroxyacyl-ACP, and phaG use
5 KB (748 words) - 02:49, 2 November 2017
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...2017.igem.org/Team:BNU-China/Collaborations "/> BNU-China</a> helped us to construct a PSB-assisted phytoremediation dynamic system mathematical model for the i
<h2>Construct an alliance to build a worldwide database</h2>
17 KB (2,383 words) - 01:53, 2 November 2017
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<p style="font-size:30px">For the whole circuit construct, we did following experiments:</p>
AgrA, which is an essential protein in quorum sensing construct, signifies toxicity to cells during growth.
10 KB (1,056 words) - 13:20, 1 December 2017
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...escription">With the rapid development of synthetic biology, we propose to construct a biosensor system for AFT detection. Saccharomyces cerevisiae is chosen as
...e may not be suitable for commercial glucometer detection. So we transfect construct that can constitutively express HXT2 to JDYY001, and cultured them in the l
31 KB (3,893 words) - 01:49, 2 November 2017
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...sed our engineered strain pMC( strain co-expressed Mtr CAB and Ccm A-H) to construct a bio-cathode. We monitored the current of the bio-cathode to see whether t
...se the efficiency of electron transferring. Then what we need to do was to construct this bio-cathode, put every part of this “toy” together and get the oxy
21 KB (2,589 words) - 10:23, 30 October 2017
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...f testing for iron.</p><div>In order to react to different iron levels our construct will be promoted by the AceB gene, which can be regulated by FUR, a transcr
2 KB (372 words) - 15:07, 30 October 2017
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...iments of the bio-cathode, as most of our team members did not know how to construct and measure all kinds of properties of a bio-cathode, Dr.Liu gave us a grea
...iments of the bio-cathode, as most of our team members did not know how to construct and measure all kinds of properties of a bio-cathode, Dr.Tang gave us a gre
27 KB (2,696 words) - 13:00, 1 November 2017
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...ination with GFP to switch from an [ON] to [OFF] state, we have produced a
construct where in the [ON] state the non-fluorescense based reporter protein eforRed
<p class="FIM">To
construct the Fim reporter switch 3 separate gBlocks were designed with overlapping a
18 KB (2,575 words) - 09:48, 23 October 2017
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...the pBS1C vector. Thus, positive integration of the pBS1C-SPM-<i>amyE</i> construct lead to white colonies, when plated on X-Gal containing agar plates. (Figur
...more potent than the wild type. We explain this, by the way we cloned our construct: due to the RFC25 we by default added two amino acids (AA) (from the scar s
49 KB (8,021 words) - 16:10, 13 December 2017
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...sp; Focusing on tumor - targeting therapies, we wanted to construct a positive bacterium for targeting drug carriers to increase the efficiency
...ze3"> We did a pre experiment about the construct of a positive bacterium, and the results showed that the experimental proje
5 KB (695 words) - 13:51, 1 November 2017
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...te which prevents network formation. This inhibition site is linked to the construct with a linker which contains a specific protease cleavage site. Proteases e
12 KB (1,889 words) - 15:24, 1 November 2017
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<a href="#
Construct" style="font-size: 14px;">
Construct validation</a>
<div id="
Construct">
35 KB (4,821 words) - 03:39, 2 November 2017
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to construct stable cell lines. Based on these cell lines, we infected different
virus to construct a super cell line, which has a high expression of the three genes.
27 KB (2,059 words) - 09:44, 30 October 2017
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<p>without compromising the function of the construct that is originally designed. Such a system has the potential uses on both s
...s a transcriptionally active complex with 3OC6HSL to activate a downstream construct. Once activated, the desired functions of the circuit will run along with a
11 KB (1,481 words) - 15:25, 25 October 2017
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...enetic parts are produced commercially. We use homologous recombination to
construct our entire network.</li>
<p>Despite using dCas9 to
construct a NOR gate, no gene drive is created. By following the due process, we were
10 KB (1,542 words) - 23:19, 31 October 2017
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First transfection into SF-21 cells with MAYV-F and MAYV-D construct.
...ts. New infection for production of MAYV-VLP’s in Sf-9. The new pFastBac construct was checked using restriction analysis and sent for sequencing.
6 KB (960 words) - 13:01, 31 October 2017
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...AWB. (Moreover, in order to indicate where the target neurons are, we also construct a fusion reporter protein called mCherry. At the same time, the fusion prot
...hodopsins and indicator in specific neuron, such as AWA, we use Podr-10 to construct the plasmid.
9 KB (1,302 words) - 16:40, 1 November 2017
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...YFP was cloned into the vector using TAR. This lead to the following final construct in the yeast cells, as depicted in figure 1.
Figure 2: Final construct of Ddx4-YFP in pYES2-CT. <br> <br>
6 KB (723 words) - 18:18, 1 November 2017
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<li>Construct 3hree vectors producing dsRNA (Ecr-L4440, ECRL, ECRN)</li>
<li>Construct vectors of dsRNA which act on arabidopsis thaliana.</li>
22 KB (2,569 words) - 13:49, 1 November 2017
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...ach our goal, we select two methods, co-expression and surface display. To construct the co-expression system, the gene of fluorescent protein---smURFP and the
To construct the surface presentation system, the gene of fluorescent protein---smURFP a
8 KB (971 words) - 18:09, 1 November 2017
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time to construct it. This could be a plasmid we design for future USYD
iGEM teams to construct.
42 KB (5,797 words) - 02:16, 2 November 2017
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...oxicity from potential. We included a potential riboswitch in the BioBrick construct because it allowed the cyanobacteria to stop producing tagatose when concen
...to HPLC because it was simpler and more convenient.<b> We also changed the construct of the BioBrick by introducing a flag tag </b> . In the event that tagatose
42 KB (6,166 words) - 01:43, 2 November 2017
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The first construct containing the genes required for the light-induced dormancy was designed a
...ite between the LacI-regulated, lambda hybrid promoter and this particular construct under the tested conditions had formed a hotspot for transposons. In the li
79 KB (11,411 words) - 01:33, 2 November 2017
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...stem based on CpxR-CpxR dimerization (Figure 1A) was the most promising to construct in the lab. This is likely because one activated CpxA can amplify its signa
...with BiFC is indeed a viable option. Because we put the CpxR-eYFP-termini construct under control of the inducible araC/pBAD promoter we were able to test hypo
22 KB (2,393 words) - 13:13, 29 October 2017
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...tists to calibrate the defect in engineered biological construct, to share construct between labs and to analyse experimental results across different lab condi
11 KB (1,237 words) - 05:14, 3 December 2017
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...ed a way to quickly and easily determine if a given genetically engineered construct is actually producing fumarate. For this purpose, we constructed a biosenso
...hen bind to DNA at specific loci to initiate a biological response [2]. We construct a fumarate biosensor based on the DcuSZ/OmpR chimeric TCS developed in
44 KB (5,388 words) - 15:35, 15 December 2017
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background, to construct the
...he cyclic nature of the TCA, which may no longer operate as a cycle. This construct yields one fumarate per glycogen catabolized. We started wondering if there
106 KB (12,576 words) - 19:52, 15 December 2017
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...and help. We also gave team SMS_Shenzhen tips on how to document parts and construct the part wikis and helped them with their part characterizations. <br><br><
12 KB (1,472 words) - 02:45, 30 October 2017
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...ttps://2017.igem.org/Team:UrbanTundra_Edmonton/Design">gBlocks</a> for our construct (PcrA, PcrB, LacIQ).</p>
...Allowing us to do our diagnostic digestions and gel electrophoresis of our construct in their lab<br>Troubleshooting our transformations</p>
5 KB (509 words) - 02:51, 2 November 2017
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...our theoretical design, no tests have been performed to validate a working
construct yet (Ability to bind to cellulose, ect.)</p>
<p>Part BBa_K2522001 is a working
construct developed to produce Super Folder Green Fluorescent Protein in the presence
4 KB (597 words) - 22:50, 1 November 2017
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<p style="text-align: center;font-family:'Open Sans', sans-serif">Fig The construct map of pDG1730</p>
...the varied activity of the four Agr types, we transformed the pLI50-P2-GFP construct from RN4220 to Newman(Agr typeⅠ), N315 (Agr type Ⅱ). We found that the
24 KB (3,512 words) - 16:01, 26 October 2017
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.../a>). For the verification of the system, we designed the characterization construct shown in Figure 1, and we named it as pET23a-TA.</p>
...we could also induce the expression of CbtA (toxin) by L-arabinose. So the construct can be simplified into Figure 2. We can know from the structure that the ad
18 KB (2,666 words) - 17:44, 1 November 2017
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<p>2015 Peking iGEM Team: </span>Inspired us on our construct design.</p>
...p>Integrated DNA Technologies (IDT): Provided us with parts of DNA for our construct.</p>
79 KB (7,597 words) - 16:06, 1 November 2017
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...gle-stranded functionalized DNA capable of cleaving a fluorophore-quencher construct specifically in the presence of E. coli. Upon cleavage, the quencher can no
...gle-stranded functionalized DNA capable of cleaving a fluorophore-quencher construct specifically in the presence of E. coli. Fluorescence was chosen for its hi
10 KB (1,595 words) - 06:25, 31 October 2017
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...the pBS1C vector. Thus, positive integration of the pBS1C-SPM-<i>amyE</i> construct lead to white colonies, when plated on X-Gal containing agar plates. (Figur
...more potent than the wild type. We explain this, by the way we cloned our construct: due to the RFC25 we by default added two amino acids (AA) (from the scar s
58 KB (9,491 words) - 17:50, 1 November 2017
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...help us construct our test-paper. They borrowed pet28a plasmids from us to construct their parts but could only provide us with the bacteria, but not the purifi
33 KB (4,502 words) - 03:20, 2 November 2017
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...nbsp; We chose five metal ions to construct our metal detecting module, and three of them
...nks. Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria [J]. Sensors (Basel). 2016 Dec 18;16(12).
29 KB (3,091 words) - 12:51, 1 November 2017
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<b>Fig. 2:</b> Schematic structure of our asr_mCardinal construct containing all important coding and noncoding parts.
<b>Fig. 3: </b>Schematic structure of our alx-mNeonGreen construct containing all important coding and noncoding parts.</b>
9 KB (1,233 words) - 01:55, 2 November 2017
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<h3> Construct Building and Validation </h3>
As a first quick test of our construct, we transformed pT8-O and a plasmid not containing adenylate cyclase (as a
4 KB (678 words) - 03:19, 2 November 2017
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...y parts BBa_K145201 and BBa_K081012(GFP Generator). When we expressed this construct in the high copy number vector pSB1C3, we found that a significant basal le
15 KB (1,561 words) - 00:04, 2 November 2017
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.... We modified the protein that is control to amilCP thereby creating a new construct & introducing further variability to characterised biobricks.</p>
3 KB (531 words) - 22:13, 31 October 2017
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...contain IPTG - normally, no IPTG is necessary to observe fluorescence with construct BBa_J04450, since expression is apparently leaky enough.
...ized better than they normally are. For example, bacteria with the RFP-DAS construct normally accumulate enough RFP to appear red on a LB plate - would growth i
6 KB (1,029 words) - 02:02, 2 November 2017
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<p5 class="w3-text-grey"> We liked the
construct very much, so we asked Team Aachen for the plans and the program of the sen
<p5 class="w3-text-grey">To be able to finalize our
construct, it was needed to have an effective promoter, which can be used in the bact
8 KB (1,207 words) - 00:08, 2 November 2017
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...<p class="card-text">Fig1. the PC1 laboratory in which the construct for our plant transformation was developed. </p>
...ring sterile plates in the laminar flow hood, to be used for culturing our construct in E. coli. </p>
7 KB (841 words) - 03:30, 1 November 2017
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<p>
Construct parts with Qijie Xu</p>
<p>
Construct parts with Chushu Zhu</p>
15 KB (1,797 words) - 03:24, 2 November 2017
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...nses from our collaborator HKUST. As for the General Simulation System, we construct a highly robust ODE system to characterize a wide range of genetic circuits
22 KB (2,794 words) - 17:01, 20 November 2017
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<h3>Why do we construct this App?</h3>
<h3>How do we construct this App?</h3>
7 KB (896 words) - 14:23, 1 November 2017
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Finalized construct design.
Design GeneBlocks in order to construct the big QS ligation and as soon as they arrive: start ligation.
12 KB (1,971 words) - 13:06, 31 October 2017
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.../a>). For the verification of the system, we designed the characterization construct shown in Figure 1, and we named it as pET23a-TA.</p>
...we could also induce the expression of CbtA (toxin) by L-arabinose. So the construct can be simplified into Figure 2. We can know from the structure that the ad
18 KB (2,667 words) - 17:09, 1 November 2017
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<br><h6>Figure 1. Pthalate Reporter Construct</h6>
<br><h6>Figure 2. Salicylate Reporter Construct</h6>
3 KB (442 words) - 03:58, 2 November 2017
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...tech Co. and fused to a reporter gene mCherry and rrnB T1 terminator. This construct was cloned into pLEV1 vector to result to pLEV1-mCherry vector. Site-direct
22 KB (2,974 words) - 02:52, 2 November 2017
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...ze variation of a colony PCR product on an agarose gel because the overall construct is still short. To observe a hundred-base pair variation in a 3 or 4 kb con
...other experiments like, for example, the insertion of the UBC into another construct, with a simple PCR reaction.</p>
13 KB (1,842 words) - 10:46, 30 November 2017
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...nfortunately, we can’t. Therefore, we cannot choose C4HSL and 3OC6HSL to construct the two warriors that we want. <br>
...tioned in Fig. 5. In other words, the best design we can achieve now is to construct one warrior that can be only killed by the other . Of course, the circuit i
16 KB (2,320 words) - 15:54, 1 November 2017
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<p5 class="w3-text-grey"> We liked the
construct very much, so we asked Team Aachen for the plans and the program of the sen
<p5 class="w3-text-grey">To be able to finalize our
construct, it was needed to have an effective promoter, which can be used in the bact
8 KB (1,207 words) - 00:05, 2 November 2017
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...an unstable growth of cell, thus, the choice of anchor unit is the key to construct the surface display system. Furthermore, INP surface display system has the
...y) did not have the correct function as we expect. Therefore, we decide to construct the adhesion platform.
33 KB (4,400 words) - 13:55, 18 November 2017
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The sequencing data came back and we worked out that the construct was heavily mutated by the ''E. coli''. This meant that the constructs must
...n order to work out our plasmid concentrations. This was also to see which construct needed more minipreps.
13 KB (1,726 words) - 23:11, 31 October 2017
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...rator (Part: BBa_K145201), Ptet promoter and a GFP generator. We made this construct since we wanted to test and optimize the induction of the pTet promoter. In
13 KB (1,328 words) - 02:46, 2 November 2017
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...y parts BBa_K145201 and BBa_K081012(GFP Generator). When we expressed this construct in the high copy number vector pSB1C3, we found that a significant basal le
15 KB (1,561 words) - 02:48, 2 November 2017
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...rator (Part: BBa_K145201), Ptet promoter and a GFP generator. We made this construct since we wanted to test and optimize the induction of the pTet promoter. In
13 KB (1,328 words) - 22:21, 1 November 2017
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<p>To
construct the autoinduction, AIP sender, and AIP receiver system in <i>L. lactis</i>,
<p>We originally planned to
construct the AIP receiver or autoinduction systems by cloning agrC-agrA, or argBDCA
28 KB (4,046 words) - 06:09, 1 November 2017
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<b>Figure 3:</b> Rose fragrance map construct, designed with SnapGene.
Our rose fragrance construct is shown in Figure 12. The assembled rose plasmids were sucessfully transfo
9 KB (1,264 words) - 22:40, 14 December 2017
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...o regulate the water quality. For the sake of mobility and portability, we
construct this device into a boat, which can move around to collect data and is conve
<p>We did a lot of experiments to
construct and test the function of our bricks. All of the laboratory tests get good r
8 KB (1,239 words) - 09:10, 1 November 2017
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...a>). IDT performed the DNA synthesis and delivered the part as gBlock. The construct was cloned by conventional ligation into the pSB1C3 plasmid and transformed
The size of the integrated construct should be 2119 bp. Note that the DNA Ladder they used (MassRuler DNA Ladder
35 KB (4,398 words) - 20:38, 1 November 2017
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...r future work, we will run numerous sets of <i>E. coli</i> kill curve with construct BBa_K2221002 and plot a baseline-corrected graph of GlmY+J23102 promoter. A
8 KB (1,198 words) - 03:54, 2 November 2017
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<p> In addition, once the Ms2-ASO
construct reaches the splice-site, it will provide an extra barrier to reaching the s
<p> It is especially important to check the secondary structure of this ASO
construct. We need to maintain the hairpin loop while also leaving the ASO open to bi
9 KB (1,461 words) - 02:36, 2 November 2017
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Construct expression system for T7 PC report system and Fluc PC report system. </br>
</br></br>We construct both two parts for T7 PC report system and Fluc PC report system. Fluc PC r
20 KB (2,964 words) - 12:55, 1 November 2017
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...dless</b> implications of this method and thus decided to incorporate this construct into our yeast system. </p>
...cessful because our colonies were pink. This was indicative of the mCherry construct being present, a region of the plasmid we were trying to remove.</p>
17 KB (2,577 words) - 01:53, 4 December 2017
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...ssette in the middle of the upstream and downstream homologous regions. To construct these two plasmids as convenient as possible, we adopted a fusion-PCR based
Fusion-PCR based strategy to construct two plasmids required for markerless genetic modification. H1 and H2 were a
29 KB (3,502 words) - 09:31, 23 November 2017
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polycistronic construct under the control of a strong promoter.
...from the iGEM distribution kit 2017 BBa_J2310 we used this new construct to transformed E. coli competent DH5α.
23 KB (3,109 words) - 20:32, 18 December 2017
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<b>Figure 1: Snapgene overview of NTHU Taiwan’s
construct.</b>
<p>TU Delft offered to revise their SnapGene files featuring their
construct to discard a faulty design as the issue and shared a series of recommendati
22 KB (3,027 words) - 21:48, 14 December 2017
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...t:16px;">Because organisms do not naturally synthesize tagatose, we had to construct the metabolic pathway for this reaction to occur. We used a number of sourc
...style="margin-left:16px;">After we were done with each individual BioBrick construct and validated the enzymes via DNA sequencing and confirmation, we then deci
12 KB (1,715 words) - 02:05, 1 November 2017
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...rse aligned well with the T7 flanks, and the first half of the protospacer construct. However, a large gap in alignment was seen spanning from the end of protos
...</strong>Colony 1 sequences indicated a loss of two protospacers from the construct. Diagram created by modifying image produced by sequence analysis tool on B
5 KB (776 words) - 23:48, 1 November 2017
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<p>We tried to construct four inserts in total for our project. “INSERT1” is for lac I synthesis
...bond formation respectively between linkers and cysteine modules. The part construct is quite like that of part 2. The only difference is that part 3 lacks SAP
3 KB (528 words) - 00:32, 2 November 2017
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cloning procedure for our synthetically designed promoter construct.</span></b></p>
14.0pt;line-height:150%;font-family:"Times New Roman","serif"'>The construct formations
35 KB (4,521 words) - 22:49, 1 November 2017
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plasmid we construct, we manage to bind our enzyme on gauze. This specific design excels in 3
we construct plasmid and manage to express the enzyme that able to bind with gauze(made
25 KB (3,111 words) - 03:25, 2 November 2017
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Construct OD600 reference point<br/>
Construct fluorescence standard curve
21 KB (3,184 words) - 01:29, 2 November 2017
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...rse. However, we had performed functional test previously with the missing construct, and have used the tag successfully on different constructs, such as our J2
...olor: #808080; font-size: 14px;'>Figure 1: Schematic of a generic reporter construct used to test degradation rates. Analogous constructs were built with sfGFP
4 KB (548 words) - 03:32, 2 November 2017
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... Repressilator was first
construct by Michael B.
...><p class="scu_content_attri"> We bought well-
construct
41 KB (3,870 words) - 21:04, 1 November 2017
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....org/Team:ICT-Mumbai/Description"><mark>Description</mark></a>, we wish to construct <i>Escherichia coli</i> cells that can assimilate ammonia and convert it in
<h5 class="w3-center" style="color:#000080">Final construct...</h5>
13 KB (1,837 words) - 22:30, 1 November 2017
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<li> Cells with stable integration of our ToleGen construct, this means a copy number of 1. </li>
...degradation rate, Cre stably associate with the target site in the product construct). </li>
13 KB (1,455 words) - 00:46, 2 November 2017
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...for the full sequence of mRFP, a red fluorescent protein, above 37°C. The construct is composed of a cI857 coding gene behind the strong constitutive promoter
...above 30°C until 42°C, when it can no longer bind to pL. The aim of this construct is to code for mRFP when the temperature rises above 30°C, while the trans
25 KB (3,703 words) - 00:32, 2 November 2017
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...Sarich</a>). The issues raised were not with the design of the kill switch construct or its ability to cause lethality in the lab, but with implementation into
...towards closed, industrial settings, we wanted to learn about views of our construct in those settings. To learn more about lab culture and determine how that m
22 KB (3,410 words) - 01:21, 2 November 2017
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<p5 class="w3-text-grey"> We liked the
construct very much, so we asked Team Aachen for the plans and the program of the sen
<p5 class="w3-text-grey">To be able to finalize our
construct, it was needed to have an effective promoter, which can be used in the bact
8 KB (1,207 words) - 00:06, 2 November 2017
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...ed a way to quickly and easily determine if a given genetically engineered construct is actually producing fumarate. For this purpose, we constructed a biosenso
...hen bind to DNA at specific loci to initiate a biological response [2]. We construct a fumarate biosensor based on the DcuSZ/OmpR chimeric TCS developed in
38 KB (4,709 words) - 21:15, 1 November 2017
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...ssette in the middle of the upstream and downstream homologous regions. To construct these two plasmids as convenient as possible, we adopted a fusion-PCR based
Fusion-PCR based strategy to construct two plasmids required for markerless genetic modification. H1 and H2 were a
27 KB (3,270 words) - 20:12, 1 November 2017
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...ssette in the middle of the upstream and downstream homologous regions. To construct these two plasmids as convenient as possible, we adopted a fusion-PCR based
Fusion-PCR based strategy to construct two plasmids required for markerless genetic modification. H1 and H2 were a
27 KB (3,314 words) - 22:23, 1 November 2017
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...ed a way to quickly and easily determine if a given genetically engineered construct is actually producing fumarate. For this purpose, we constructed a biosenso
...hen bind to DNA at specific loci to initiate a biological response [2]. We construct a fumarate biosensor based on the DcuSZ/OmpR chimeric TCS developed in
39 KB (4,799 words) - 22:23, 1 November 2017
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...r. We would test this by looking at the level of induction needed from our construct to reduce the fluorescence. This would allow a more accurate figure for our
<h6 class="text-center">We would then construct a plasmid with TEV protease under the tet operator and promoter, and add th
15 KB (2,395 words) - 03:59, 2 November 2017
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...ssette in the middle of the upstream and downstream homologous regions. To construct these two plasmids as convenient as possible, we adopted a fusion-PCR based
Fusion-PCR based strategy to construct two plasmids required for markerless genetic modification. H1 and H2 were a
27 KB (3,289 words) - 23:14, 1 November 2017
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...ed a way to quickly and easily determine if a given genetically engineered construct is actually producing fumarate. For this purpose, we constructed a biosenso
...hen bind to DNA at specific loci to initiate a biological response [2]. We construct a fumarate biosensor based on the DcuSZ/OmpR chimeric TCS developed in
38 KB (4,724 words) - 23:55, 1 November 2017
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<a class="active item passlink" data-passage="consvec">Construct the Expression Vectors</a>
<h1>Construct the Expression Vectors</h1>
31 KB (4,295 words) - 13:26, 15 December 2017
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...site Bba_B0034 and double terminator Bba_B0015 are also integrated to this construct.<br />Ref: Diversity and Evolution of Coral Fluorescent Proteins,Naila O. A
...site Bba_B0034 and double terminator Bba_B0015 are also integrated to this construct. This part expresses AmilCP in E.coli since pL promoter expresses constitut
2 KB (291 words) - 02:47, 2 November 2017
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...font-size: 0.5em;">Image 1. 40x zoom for 25ng/ul aTc concentration of HxlR construct after overnight measurement of induction.</p>
...5em;">Image 2. 40x zoom for 25ng/ul aTc and 1 mM VOC concentration of HxlR construct after overnight measurement of induction.</p>
7 KB (1,126 words) - 01:26, 2 November 2017
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...nstructs, using mCherry fluorescence to check the expression level of each construct.<br>
...microscopy images of Low, Medium and High strength Anderson promoter-PduD construct associated mCherry (OD600: 0.2) expressed in the absence of Eut.</center></
6 KB (750 words) - 03:27, 2 November 2017
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...ver and whenever needed. In the course of the project phase, the team will construct a Grätzel cell testing their dye that was produced in E. coli.
6 KB (998 words) - 22:31, 15 December 2016
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As synthetic biologists continue to construct increasingly complex gene regulatory networks, the need for accurate quanti
6 KB (903 words) - 15:12, 27 January 2017
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...dsorption, but pigments will certainly affect other factors. Therefore, we construct several models that each represents an important factor in the growth and c
59 KB (6,227 words) - 19:22, 1 November 2017
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...esigned primers. The gel electrophoresis results demonstrate our effort to construct the pigmentation genes collection. The followings are MelA, Lycopene’s up
9 KB (1,229 words) - 23:34, 1 November 2017
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...nt> by combining holin and endolysin with nitrogen affinity protein in one construct for issues regarding biosafety. We successfully proved the function of nitr
11 KB (1,233 words) - 11:07, 30 October 2017
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...nt> by combining holin and endolysin with nitrogen affinity protein in one construct for issues regarding biosafety. We successfully proved the function of nitr
1 KB (184 words) - 16:00, 1 November 2017
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<li> Debug a construct </li>
2 KB (329 words) - 18:59, 26 March 2018
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<li> Debug a construct </li>
2 KB (329 words) - 17:07, 22 February 2017
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The kit includes three vials of purified plasmid DNA from BBa_J04450 (RFP construct) in plasmid backbone pSB1C3. Each vial contains 50 µL of DNA at a differen
8 KB (1,132 words) - 19:37, 17 May 2017
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for our project modeling to construct the pathway.
11 KB (1,138 words) - 18:14, 1 November 2017
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...the crRNA, is used by dCas9 to interfere with the expression of a reporter construct. If a match is found, our dCas9 will target and bind to one of the corresp
22 KB (3,327 words) - 02:46, 2 November 2017
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...y promoter to activate our reporter genes known as PMC1 which we worked to construct in the wet lab.
10 KB (1,365 words) - 17:42, 12 December 2017
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The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E
9 KB (1,094 words) - 20:16, 10 August 2017
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...n:</b> Made and ran gel testing to test the presence of the correct gibson construct which failed. Worked on universal promoter wiki and began work on modifying
93 KB (14,733 words) - 21:06, 14 October 2017
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In this project, we aim to construct a set of artificial RNA biosensors to detect different influenza A viral ge
15 KB (2,265 words) - 20:28, 1 November 2017
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...We have more recently been attempting to sequence this to ensure that the construct is intact and as expected before expression in BL21 (DE3) cells. Parallel t
4 KB (601 words) - 15:29, 30 June 2017
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...e exact concentrations required for optimal growth of the SRB’s. We will construct a salt biosensor using the osmo-responsive promoter of the ProU operon to d
17 KB (1,987 words) - 01:35, 2 November 2017
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...r biology software that is easier to use than pen and paper. Now every DNA construct made in our lab can be documented in a rich electronic...</p></td></tr></ta
28 KB (3,611 words) - 14:59, 8 June 2017
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The goal is to be able to construct afterward.
71 KB (11,499 words) - 03:55, 2 November 2017
